Detailed information of TA system TA079030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 296564..297471
• Replicon: plasmid p_unamed1
• Accession: NZ_CP022429
• Organism: Pantoea ananatis strain YJ76

Toxin (Protein)

• Gene name: -
• Uniprot ID: D4GET9
• Locus tag: B7764_RS23685
• Protein ID: WP_013027861.1
• Coordinates: 296564..297031 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: B7764_RS23690
• Protein ID: WP_014598335.1
• Coordinates: 297028..297471 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7764_RS23665	291950..292183	-	234	WP_013027857.1	DUF333 domain-containing protein	-
B7764_RS23670	292271..292843	+	573	WP_089517892.1	NAD(P)H-dependent oxidoreductase	-
B7764_RS23675	292905..293840	-	936	WP_013027859.1	hypothetical protein	-
B7764_RS23680	294525..295919	-	1395	WP_014333136.1	flagellar filament capping protein FliD	-
B7764_RS23685	296564..297031	-	468	WP_013027861.1	RES family NAD+ phosphorylase	Toxin
B7764_RS23690	297028..297471	-	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
B7764_RS23695	297857..298081	+	225	WP_013027863.1	KTSC domain-containing protein	-
B7764_RS23700	298236..298931	+	696	WP_089517893.1	hypothetical protein	-
B7764_RS23705	299007..299696	-	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
B7764_RS23710	300095..301186	+	1092	WP_013027866.1	pyrimidine utilization protein A	-
B7764_RS23715	301183..301935	+	753	WP_019105823.1	pyrimidine utilization protein B	-
B7764_RS23720	301961..302347	+	387	WP_013027868.1	pyrimidine utilization protein C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC / fliC	1..328324	328324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17207.70 Da
• Isoelectric Point: 5.1570

>T79030 WP_013027861.1 NZ_CP022429:c297031-296564 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQV

Nucleotide

• Length: 468 bp

>T79030 NZ_CP022429:c297031-296564 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACTGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTGCCTGACGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTGCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGTATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT79030 WP_014598335.1 NZ_CP022429:c297471-297028 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT79030 NZ_CP022429:c297471-297028 [Pantoea ananatis]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGTGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGCAATAAAAAACCGGCCGATCTGGTTGCGTCAGAAACCGGTGCTTATGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA