Detailed information of TA system TA079008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 1570646..1571195
• Replicon: chromosome
• Accession: NZ_CP022423
• Organism: Vitreoscilla filiformis strain ATCC 15551

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: VITFI_RS07440
• Protein ID: WP_089418028.1
• Coordinates: 1570926..1571195 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: VITFI_RS07435
• Protein ID: WP_198301671.1
• Coordinates: 1570646..1570942 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VITFI_RS07420	1566299..1568131	-	1833	WP_198301670.1	NAD(P)H-dependent oxidoreductase subunit E	-
VITFI_RS07425	1568256..1568630	-	375	WP_089416440.1	zf-TFIIB domain-containing protein	-
VITFI_RS07430	1568735..1570636	+	1902	WP_089418027.1	ATP-binding cassette domain-containing protein	-
VITFI_RS07435	1570646..1570942	-	297	WP_198301671.1	BrnA antitoxin family protein	Antitoxin
VITFI_RS07440	1570926..1571195	-	270	WP_089418028.1	BrnT family toxin	Toxin
VITFI_RS07445	1571303..1572778	+	1476	WP_089418030.1	M20 family metallopeptidase	-
VITFI_RS07450	1572738..1574354	-	1617	WP_157725598.1	adenosine deaminase	-
VITFI_RS07455	1574357..1575445	-	1089	WP_089416442.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1568872..1657829	88957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (31-93)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10193.65 Da
• Isoelectric Point: 10.2943

>T79008 WP_089418028.1 NZ_CP022423:c1571195-1570926 [Vitreoscilla filiformis]
MNFRFDLTKDAANIAKHGVSLALAAQLEWDSALVWPDTRRDYGEPRQSALVLMGVRLFFVAFVDRPEGRRIISLRKANSR
EVNRYVGQD

Nucleotide

• Length: 270 bp

>T79008 NZ_CP022423:c1571195-1570926 [Vitreoscilla filiformis]
ATGAACTTCCGCTTCGATCTCACCAAAGACGCTGCCAACATCGCCAAGCACGGTGTTTCACTGGCACTGGCGGCACAACT
GGAATGGGATTCGGCGCTGGTGTGGCCCGACACACGCCGTGACTATGGCGAGCCTCGCCAAAGTGCTCTGGTTTTGATGG
GGGTACGCTTGTTCTTCGTCGCTTTCGTGGATCGCCCTGAGGGGCGGCGCATCATCAGCCTGCGCAAAGCCAATTCAAGA
GAGGTGAACCGCTATGTTGGTCAAGACTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10830.51 Da
• Isoelectric Point: 10.3084

>AT79008 WP_198301671.1 NZ_CP022423:c1570942-1570646 [Vitreoscilla filiformis]
MLVKTKSGRLVEMPTPEDDARITAAAMEDPDALPLTDGQWQAVKPLARLGRPPAQRPLKKPTTIRFDADVLDALKATGKG
WQTRVNDAMREWVKTHAA

Nucleotide

• Length: 297 bp

>AT79008 NZ_CP022423:c1570942-1570646 [Vitreoscilla filiformis]
ATGTTGGTCAAGACTAAATCCGGGCGCTTGGTGGAAATGCCCACGCCCGAGGACGACGCACGCATCACGGCTGCGGCCAT
GGAAGACCCGGACGCACTTCCCCTGACCGATGGCCAATGGCAAGCGGTCAAACCCTTGGCACGCCTCGGGCGCCCACCGG
CGCAACGCCCGCTCAAAAAACCCACCACCATCCGCTTTGATGCCGATGTGCTGGACGCTCTCAAAGCCACCGGCAAGGGC
TGGCAAACACGGGTGAACGACGCCATGCGTGAGTGGGTGAAAACCCACGCGGCATGA