Detailed information of TA system TA079005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 81091..81703
• Replicon: plasmid pSMR1-7
• Accession: NZ_CP022422
• Organism: Sulfitobacter pseudonitzschiae strain SMR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SULPSESMR1_RS24790
• Protein ID: WP_089423658.1
• Coordinates: 81091..81486 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SULPSESMR1_RS24795
• Protein ID: WP_089423659.1
• Coordinates: 81476..81703 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SULPSESMR1_RS24780	78841..79908	-	1068	WP_198362935.1	hypothetical protein	-
SULPSESMR1_RS24785	80136..81029	+	894	WP_089423657.1	recombinase family protein	-
SULPSESMR1_RS24790	81091..81486	-	396	WP_089423658.1	PIN domain-containing protein	Toxin
SULPSESMR1_RS24795	81476..81703	-	228	WP_089423659.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SULPSESMR1_RS24800	81941..83128	+	1188	WP_055687614.1	plasmid partitioning protein RepA	-
SULPSESMR1_RS24805	83125..84141	+	1017	WP_089423660.1	plasmid partitioning protein RepB	-
SULPSESMR1_RS24810	84319..85539	+	1221	WP_089423661.1	replication initiation protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92794	92794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-46)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14238.24 Da
• Isoelectric Point: 4.1547

>T79005 WP_089423658.1 NZ_CP022422:c81486-81091 [Sulfitobacter pseudonitzschiae]
MSAEFADTNVVLYLLDDGPKADRAEDILGQGPRISVQVLNEAMVNCRRKAGLSWEETGAFLAGVQSLCPVEDLSVQTHLV
GRALAEKYQLSVYDAMIVAAALIAGCTTLWSEDMHDGLLVEDQLRVVNPFT

Nucleotide

• Length: 396 bp

>T79005 NZ_CP022422:c81486-81091 [Sulfitobacter pseudonitzschiae]
ATGAGCGCTGAATTCGCGGATACAAATGTCGTCCTTTACCTTCTTGACGACGGCCCAAAGGCGGATCGGGCGGAAGACAT
CCTGGGGCAGGGGCCGCGGATCAGTGTGCAGGTCCTGAACGAAGCGATGGTCAATTGCCGGAGGAAAGCCGGTCTCAGTT
GGGAGGAGACCGGCGCTTTTCTCGCAGGGGTCCAATCCTTGTGTCCAGTCGAGGACCTGTCTGTGCAAACCCATCTTGTG
GGCCGAGCTTTGGCAGAAAAGTACCAGCTTTCGGTCTATGACGCGATGATTGTGGCGGCCGCTCTTATTGCTGGATGCAC
GACTTTGTGGAGCGAAGATATGCACGACGGATTGTTGGTCGAAGATCAGTTGCGCGTCGTGAATCCATTCACCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8504.77 Da
• Isoelectric Point: 10.5807

>AT79005 WP_089423659.1 NZ_CP022422:c81703-81476 [Sulfitobacter pseudonitzschiae]
MQVAKWGNSLAVRLPADLVRELGLKEGDQIDLIKDDGTLLVQRQPRADEVLQGLRRFRGKLSKEARLSRDAAHER

Nucleotide

• Length: 228 bp

>AT79005 NZ_CP022422:c81703-81476 [Sulfitobacter pseudonitzschiae]
ATGCAAGTTGCAAAATGGGGGAATTCGCTCGCGGTCCGGCTGCCTGCCGATCTTGTGCGCGAACTTGGTCTTAAAGAGGG
TGACCAAATCGATCTGATAAAGGACGACGGTACTTTGCTTGTCCAGCGCCAACCTCGCGCCGATGAAGTCCTGCAAGGGC
TAAGGCGGTTTCGCGGAAAGCTATCCAAGGAAGCACGCCTGAGCCGCGACGCTGCGCATGAGCGCTGA