Detailed information of TA system TA078998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2412094..2412698
• Replicon: chromosome
• Accession: NZ_CP022411
• Organism: Pseudomonas sp. RU47

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7Z2M765
• Locus tag: CCX46_RS11025
• Protein ID: WP_038369612.1
• Coordinates: 2412405..2412698 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CCX46_RS11020
• Protein ID: WP_127926672.1
• Coordinates: 2412094..2412402 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCX46_RS11005	2408629..2410122	+	1494	WP_127926669.1	HAMP domain-containing histidine kinase	-
CCX46_RS11010	2410091..2411539	+	1449	WP_127926670.1	sigma-54 dependent transcriptional regulator	-
CCX46_RS11015	2411690..2412037	-	348	WP_127926671.1	hypothetical protein	-
CCX46_RS11020	2412094..2412402	-	309	WP_127926672.1	putative addiction module antidote protein	Antitoxin
CCX46_RS11025	2412405..2412698	-	294	WP_038369612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCX46_RS11030	2412904..2413701	+	798	WP_127926673.1	class I SAM-dependent methyltransferase	-
CCX46_RS11035	2413837..2414529	-	693	WP_127926674.1	N-acetyltransferase	-
CCX46_RS11040	2414526..2415716	-	1191	WP_127926675.1	GNAT family N-acetyltransferase	-
CCX46_RS11045	2415721..2416410	-	690	WP_127926676.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (24-89)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10656.23 Da
• Isoelectric Point: 10.9839

>T78998 WP_038369612.1 NZ_CP022411:c2412698-2412405 [Pseudomonas sp. RU47]
MNYLIRQTAVFQAWHQSVRDLRAKVAIARRIDRASTGNLGDVKSVGDGVSEMRVDVGAGYRVYFTMRNGAVIVLLAGGDK
SSQSADIRRAQKMAKEV

Nucleotide

• Length: 294 bp

>T78998 NZ_CP022411:c2412698-2412405 [Pseudomonas sp. RU47]
ATGAATTATCTTATTCGACAAACAGCCGTCTTCCAGGCTTGGCACCAATCGGTTCGAGACCTGCGGGCCAAGGTGGCCAT
CGCCCGCCGCATTGATCGGGCCTCAACGGGAAATCTCGGCGATGTTAAATCGGTGGGTGATGGCGTATCTGAAATGCGCG
TAGATGTGGGTGCAGGCTATCGGGTGTATTTCACGATGCGTAACGGTGCAGTCATCGTTCTATTGGCTGGAGGCGACAAG
TCTTCACAAAGCGCAGACATCCGACGCGCACAAAAAATGGCTAAGGAGGTTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11081.73 Da
• Isoelectric Point: 5.5740

>AT78998 WP_127926672.1 NZ_CP022411:c2412402-2412094 [Pseudomonas sp. RU47]
MSQTLTIFDVAALLDSDEAISEYLSQVLADGDNEEFLRAIGYVLKARGMTQIAKTSGMGRESLYKAFAPGAKPRFETVLK
VMHALGIDLYAQPGHAINHPVV

Nucleotide

• Length: 309 bp

>AT78998 NZ_CP022411:c2412402-2412094 [Pseudomonas sp. RU47]
ATGAGCCAGACGTTAACGATTTTCGACGTGGCAGCATTGCTGGACAGTGACGAAGCCATCAGCGAATACCTGTCCCAGGT
TCTTGCAGACGGGGATAACGAAGAGTTTCTTCGCGCAATCGGCTATGTGCTCAAGGCGCGCGGGATGACGCAGATAGCCA
AAACCTCTGGCATGGGTCGCGAAAGTCTGTACAAGGCGTTCGCGCCCGGCGCAAAGCCTCGCTTCGAAACGGTTTTGAAA
GTTATGCATGCCCTCGGCATCGATCTTTACGCACAGCCGGGGCATGCGATCAATCACCCGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A7Z2M765