Detailed information of TA system TA078995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1526202..1526825
• Replicon: chromosome
• Accession: NZ_CP022411
• Organism: Pseudomonas sp. RU47

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CCX46_RS06960
• Protein ID: WP_095049193.1
• Coordinates: 1526646..1526825 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CCX46_RS06955
• Protein ID: WP_127926175.1
• Coordinates: 1526202..1526609 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCX46_RS06930	1521798..1522568	+	771	WP_127926171.1	trans-aconitate 2-methyltransferase	-
CCX46_RS06935	1522749..1523477	-	729	WP_127926172.1	class I SAM-dependent methyltransferase	-
CCX46_RS06940	1524050..1524412	+	363	WP_127926173.1	hypothetical protein	-
CCX46_RS06945	1524489..1525748	-	1260	WP_127926174.1	type II toxin-antitoxin system HipA family toxin	-
CCX46_RS06950	1525741..1526058	-	318	WP_127930362.1	helix-turn-helix transcriptional regulator	-
CCX46_RS06955	1526202..1526609	-	408	WP_127926175.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CCX46_RS06960	1526646..1526825	-	180	WP_095049193.1	type II toxin-antitoxin system HicA family toxin	Toxin
CCX46_RS06965	1527194..1527712	+	519	WP_127926176.1	hypothetical protein	-
CCX46_RS06980	1528400..1529284	-	885	WP_064119706.1	alpha/beta hydrolase	-
CCX46_RS06985	1529601..1530293	-	693	WP_127926177.1	pseudouridine synthase	-
CCX46_RS06990	1530327..1530557	-	231	WP_127926178.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6646.93 Da
• Isoelectric Point: 11.1923

>T78995 WP_095049193.1 NZ_CP022411:c1526825-1526646 [Pseudomonas sp. RU47]
VNSRFLIGQLVADGWYLVRIKGSHHHFKHPTKPGLVTVPHPKKDLLKKTAISILQQALL

Nucleotide

• Length: 180 bp

>T78995 NZ_CP022411:c1526825-1526646 [Pseudomonas sp. RU47]
GTGAATAGCCGTTTTCTAATCGGCCAACTCGTCGCAGACGGTTGGTATCTGGTACGGATCAAAGGAAGTCACCATCACTT
CAAGCATCCGACCAAACCAGGACTGGTCACCGTGCCACATCCGAAAAAAGATCTGCTGAAGAAAACGGCCATCAGTATTT
TGCAGCAGGCGCTGCTTTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14482.53 Da
• Isoelectric Point: 4.7142

>AT78995 WP_127926175.1 NZ_CP022411:c1526609-1526202 [Pseudomonas sp. RU47]
MLYPIAISIGDDQHAWGVEVPDIPGCFSAGEDLDDAVAMAREAIEGHFEILADDGAPIPAASKLSLHVANPKYAGCAWAV
VDIDVLKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT78995 NZ_CP022411:c1526609-1526202 [Pseudomonas sp. RU47]
ATGCTCTACCCGATTGCGATTTCAATTGGCGATGACCAACACGCCTGGGGTGTAGAAGTCCCGGACATTCCTGGATGCTT
TTCCGCCGGAGAGGATCTGGACGATGCCGTCGCCATGGCTCGCGAAGCCATCGAAGGTCATTTCGAGATTCTCGCGGACG
ACGGCGCTCCCATTCCAGCGGCCAGCAAACTGAGCCTGCACGTGGCCAACCCGAAATACGCCGGTTGTGCATGGGCCGTG
GTGGATATCGATGTGCTCAAGTATTTGGGCAAGGCCCAAAAGCTCAACATCACCCTGCCGGGCCACTTGCTCAACCGCAT
TGATGAATATGTGTTGCATCACCCGGAAGAAAAAAGCCGCTCGGGTTTTCTGGCGTCGGCGGCTCTGAAAGTTTTGCAGC
AGGGTTGA