Detailed information of TA system TA078994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 875952..876760
• Replicon: chromosome
• Accession: NZ_CP022411
• Organism: Pseudomonas sp. RU47

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CCX46_RS03810
• Protein ID: WP_007911536.1
• Coordinates: 875952..876233 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCX46_RS03815
• Protein ID: WP_100846304.1
• Coordinates: 876287..876760 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCX46_RS03800	872244..874202	+	1959	WP_127925769.1	DUF1302 domain-containing protein	-
CCX46_RS03805	874348..875721	+	1374	WP_095119559.1	DUF1329 domain-containing protein	-
CCX46_RS03810	875952..876233	+	282	WP_007911536.1	type II toxin-antitoxin system HigB family toxin	Toxin
CCX46_RS03815	876287..876760	+	474	WP_100846304.1	transcriptional regulator	Antitoxin
CCX46_RS03820	876906..878825	-	1920	WP_127925770.1	methyl-accepting chemotaxis protein	-
CCX46_RS03825	879042..880520	-	1479	WP_127925771.1	glycosyltransferase family 39 protein	-
CCX46_RS03830	880551..881138	-	588	WP_127925772.1	ribosomal protein S5-alanine N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (49-87)

Antitoxin

• Cro/C1-type helix-turn-helix domain (97-152)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11044.79 Da
• Isoelectric Point: 10.2188

>T78994 WP_007911536.1 NZ_CP022411:875952-876233 [Pseudomonas sp. RU47]
MWLTIFDRASLRFESFEKLRQDWASASGWNVDRIPHSKLRLTSKKGPLDIYVFDIKKNECRVITWINTKTGTIYIKDILP
HAGYDKWWKGDVK

Nucleotide

• Length: 282 bp

>T78994 NZ_CP022411:875952-876233 [Pseudomonas sp. RU47]
TTGTGGCTAACCATCTTCGACAGAGCATCGCTGCGCTTCGAGTCGTTTGAGAAGCTTCGTCAAGACTGGGCCAGCGCCTC
AGGTTGGAACGTTGATCGTATCCCTCACTCGAAGCTGAGGCTGACGAGCAAGAAAGGTCCGCTTGATATTTACGTTTTTG
ATATCAAGAAAAACGAGTGCCGTGTCATTACGTGGATCAACACGAAGACTGGCACTATCTATATCAAAGACATTCTTCCC
CATGCCGGATATGACAAATGGTGGAAGGGTGATGTCAAGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17491.88 Da
• Isoelectric Point: 4.6243

>AT78994 WP_100846304.1 NZ_CP022411:876287-876760 [Pseudomonas sp. RU47]
MKRNKVEATGLDSFIADISPMLEGLGDQLATLSKLLSACHDPIKNDDDLQVRMALIDELYSHASTEDHAAARFAEFVADR
VYEYENETVLIAYSSQSEALAFLLSERGVKQKDLSEIATQSAVSEILNNKRKMTVAQIKGFAEFFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT78994 NZ_CP022411:876287-876760 [Pseudomonas sp. RU47]
ATGAAGCGTAATAAAGTCGAAGCGACAGGACTGGATTCCTTCATAGCCGATATTTCACCCATGCTTGAGGGACTGGGCGA
TCAGCTAGCGACTTTGTCCAAGTTGTTGTCTGCCTGCCATGATCCGATAAAAAATGATGACGATCTGCAAGTGCGAATGG
CCCTGATTGATGAGCTTTATTCCCACGCAAGCACCGAGGATCATGCTGCGGCACGATTTGCGGAGTTTGTGGCTGATCGG
GTTTATGAGTACGAGAATGAAACGGTGTTGATTGCTTATTCGTCCCAATCTGAAGCGCTGGCGTTTCTGCTGTCGGAAAG
AGGCGTCAAACAGAAGGACTTGTCTGAGATCGCAACTCAGAGTGCTGTTTCAGAGATATTGAATAACAAGCGAAAAATGA
CCGTTGCACAGATAAAGGGATTCGCCGAGTTCTTTAAAGTGCCGGTCGAGTTTTTTATGCACGGTGTGGTTTGA