Detailed information of TA system TA078993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 474462..475051
• Replicon: chromosome
• Accession: NZ_CP022411
• Organism: Pseudomonas sp. RU47

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7Z2RAN7
• Locus tag: CCX46_RS02145
• Protein ID: WP_038360087.1
• Coordinates: 474462..474767 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CCX46_RS02150
• Protein ID: WP_038360089.1
• Coordinates: 474764..475051 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCX46_RS02125	470262..471329	+	1068	WP_127925535.1	uroporphyrinogen decarboxylase	-
CCX46_RS02130	471508..472782	-	1275	WP_122599397.1	MFS transporter	-
CCX46_RS02135	472901..473803	+	903	WP_016983863.1	LysR family transcriptional regulator	-
CCX46_RS02140	473908..474345	-	438	WP_127925536.1	excinuclease	-
CCX46_RS02145	474462..474767	+	306	WP_038360087.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCX46_RS02150	474764..475051	+	288	WP_038360089.1	putative addiction module antidote protein	Antitoxin
CCX46_RS02155	475109..475912	-	804	WP_038360091.1	hypothetical protein	-
CCX46_RS02160	475938..477164	-	1227	WP_127925537.1	beta-ketoacyl-ACP synthase	-
CCX46_RS02165	477164..477892	-	729	WP_122599393.1	3-oxoacyl-ACP reductase FabG	-
CCX46_RS02170	477885..478349	-	465	WP_127925538.1	hotdog family protein	-
CCX46_RS02175	478346..479542	-	1197	WP_127925539.1	beta-ketoacyl-[acyl-carrier-protein] synthase family protein	-
CCX46_RS02180	479539..480021	-	483	WP_127925540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11507.17 Da
• Isoelectric Point: 9.6535

>T78993 WP_038360087.1 NZ_CP022411:474462-474767 [Pseudomonas sp. RU47]
MTEILRSSTFSSWLLKLADSRARMRIQVRIDRMADGNFGDVKAIGEGLSEVRIDYGPGYRVYFMQQGRQVVILLCGGDKS
SQPRDIKQARLIAESWQEQNP

Nucleotide

• Length: 306 bp

>T78993 NZ_CP022411:474462-474767 [Pseudomonas sp. RU47]
ATGACTGAGATTCTTCGAAGTTCCACATTTTCCAGCTGGCTTTTAAAGTTGGCTGATAGTCGCGCCAGGATGCGTATTCA
GGTGCGAATTGATCGTATGGCTGACGGAAATTTTGGCGATGTCAAAGCTATCGGTGAAGGGCTCAGCGAAGTCAGAATTG
ACTATGGCCCCGGATATCGAGTCTATTTCATGCAACAAGGTCGGCAAGTGGTCATCCTCCTTTGCGGCGGAGACAAGAGC
AGCCAACCCCGAGACATCAAGCAAGCCAGGCTCATCGCGGAGTCCTGGCAGGAGCAAAACCCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10372.76 Da
• Isoelectric Point: 4.8483

>AT78993 WP_038360089.1 NZ_CP022411:474764-475051 [Pseudomonas sp. RU47]
MTEKFTRWDSAEYLKTEEDMANYLDACMEEAGDDPAFIAKALGTIARARGMTQVARDAGLSRESLYRALSGEGNPEFGTI
LKVVKALGLKLHAST

Nucleotide

• Length: 288 bp

>AT78993 NZ_CP022411:474764-475051 [Pseudomonas sp. RU47]
ATGACCGAAAAATTCACTCGTTGGGACTCAGCCGAGTACCTCAAGACAGAAGAGGACATGGCTAACTATCTGGACGCCTG
CATGGAGGAGGCTGGAGATGACCCGGCTTTTATCGCCAAGGCACTTGGCACCATAGCGAGAGCGCGAGGCATGACTCAGG
TCGCACGTGACGCAGGGTTGTCCCGAGAGAGCCTCTACCGCGCACTATCAGGTGAAGGAAACCCCGAGTTCGGAACAATC
CTGAAAGTGGTGAAGGCGTTAGGATTGAAGTTACACGCCAGTACTTGA

Structures

Toxin

• AlphaFold DB: A0A7Z2RAN7