Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18910..19553 Replicon plasmid punamed1
Accession NZ_CP022408
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag CGC46_RS28855 Protein ID WP_001044768.1
Coordinates 18910..19326 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag CGC46_RS28860 Protein ID WP_001261287.1
Coordinates 19323..19553 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS28830 14488..14871 + 384 WP_101329730.1 hydrogenase -
CGC46_RS28835 14889..17138 + 2250 Protein_19 hypothetical protein -
CGC46_RS28840 17194..18407 + 1214 WP_164502361.1 IS3 family transposase -
CGC46_RS28855 18910..19326 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
CGC46_RS28860 19323..19553 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CGC46_RS28880 20113..20526 + 414 WP_000465041.1 hypothetical protein -
CGC46_RS28885 20528..21310 + 783 WP_001164205.1 site-specific integrase -
CGC46_RS28890 21483..21836 + 354 WP_000864810.1 colicin M immunity protein -
CGC46_RS29780 21844..22035 - 192 Protein_26 lipid II-degrading bacteriocin -
CGC46_RS29785 22039..22182 - 144 Protein_27 transcriptional regulator -
CGC46_RS28900 22249..23688 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / toxB / espP 1..81950 81950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T78990 WP_001044768.1 NZ_CP022408:c19326-18910 [Escherichia coli O121:H19]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T78990 NZ_CP022408:c19326-18910 [Escherichia coli O121:H19]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT78990 WP_001261287.1 NZ_CP022408:c19553-19323 [Escherichia coli O121:H19]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT78990 NZ_CP022408:c19553-19323 [Escherichia coli O121:H19]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References