Detailed information of TA system TA078989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5250164..5250789
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CGC46_RS27845
• Protein ID: WP_000911330.1
• Coordinates: 5250391..5250789 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CGC46_RS27840
• Protein ID: WP_000450524.1
• Coordinates: 5250164..5250391 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS27815	5245967..5246437	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CGC46_RS27820	5246437..5247009	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CGC46_RS27825	5247155..5248033	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CGC46_RS27830	5248050..5249084	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CGC46_RS27835	5249297..5250010	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CGC46_RS27840	5250164..5250391	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CGC46_RS27845	5250391..5250789	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CGC46_RS27850	5250936..5251799	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CGC46_RS27855	5251814..5253829	+	2016	WP_000829325.1	tRNA cytosine(34) acetyltransferase TmcA	-
CGC46_RS27860	5253903..5254600	+	698	Protein_5110	esterase	-
CGC46_RS27865	5254710..5254910	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T78989 WP_000911330.1 NZ_CP022407:5250391-5250789 [Escherichia coli O121:H19]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T78989 NZ_CP022407:5250391-5250789 [Escherichia coli O121:H19]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT78989 WP_000450524.1 NZ_CP022407:5250164-5250391 [Escherichia coli O121:H19]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT78989 NZ_CP022407:5250164-5250391 [Escherichia coli O121:H19]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33