Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4777019..4777679 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag CGC46_RS25500 Protein ID WP_000244778.1
Coordinates 4777266..4777679 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CGC46_RS25495 Protein ID WP_000354046.1
Coordinates 4777019..4777285 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS25470 4772307..4773050 + 744 WP_000951948.1 SDR family oxidoreductase -
CGC46_RS25475 4773107..4774540 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
CGC46_RS25480 4774585..4774896 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
CGC46_RS25485 4775060..4775719 + 660 WP_000250274.1 hemolysin III family protein -
CGC46_RS25490 4775796..4776776 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CGC46_RS25495 4777019..4777285 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CGC46_RS25500 4777266..4777679 + 414 WP_000244778.1 protein YgfX Toxin
CGC46_RS25505 4777713..4778234 - 522 WP_001055874.1 flavodoxin FldB -
CGC46_RS25510 4778346..4779242 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CGC46_RS25515 4779266..4779976 + 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CGC46_RS25520 4779982..4781715 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T78986 WP_000244778.1 NZ_CP022407:4777266-4777679 [Escherichia coli O121:H19]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T78986 NZ_CP022407:4777266-4777679 [Escherichia coli O121:H19]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT78986 WP_000354046.1 NZ_CP022407:4777019-4777285 [Escherichia coli O121:H19]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT78986 NZ_CP022407:4777019-4777285 [Escherichia coli O121:H19]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References