Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4688558..4689356 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3W5Y7S6
Locus tag CGC46_RS24990 Protein ID WP_000854916.1
Coordinates 4688558..4688935 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W5Y7S1
Locus tag CGC46_RS24995 Protein ID WP_024174207.1
Coordinates 4688982..4689356 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS24960 4684220..4684933 - 714 Protein_4570 DUF4942 domain-containing protein -
CGC46_RS24965 4685009..4685683 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
CGC46_RS24970 4685680..4686027 + 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
CGC46_RS24975 4686047..4687603 + 1557 WP_001066419.1 IS66 family transposase -
CGC46_RS29985 4687621..4687779 - 159 WP_000014512.1 hypothetical protein -
CGC46_RS24980 4687864..4688061 - 198 WP_000839246.1 DUF957 domain-containing protein -
CGC46_RS24985 4688073..4688561 - 489 WP_000777666.1 hypothetical protein -
CGC46_RS24990 4688558..4688935 - 378 WP_000854916.1 TA system toxin CbtA family protein Toxin
CGC46_RS24995 4688982..4689356 - 375 WP_024174207.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CGC46_RS25000 4689519..4689740 - 222 WP_000692312.1 DUF987 domain-containing protein -
CGC46_RS25005 4689809..4689943 - 135 Protein_4580 hypothetical protein -
CGC46_RS25010 4689984..4691181 + 1198 Protein_4581 IS3 family transposase -
CGC46_RS25015 4691259..4691609 - 351 WP_000624681.1 IS66 family insertion sequence element accessory protein TnpB -
CGC46_RS25020 4691606..4692031 - 426 WP_000422707.1 IS66 family insertion sequence hypothetical protein -
CGC46_RS25025 4692163..4692516 - 354 Protein_4584 transposase -
CGC46_RS29685 4692554..4693020 + 467 Protein_4585 transposase -
CGC46_RS29690 4693025..4693294 - 270 Protein_4586 transposase -
CGC46_RS25035 4693536..4693778 + 243 WP_096855415.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4682718..4700516 17798
- inside IScluster/Tn - - 4682718..4698048 15330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14033.04 Da        Isoelectric Point: 9.1378

>T78985 WP_000854916.1 NZ_CP022407:c4688935-4688558 [Escherichia coli O121:H19]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ

Download         Length: 378 bp

>T78985 NZ_CP022407:c4688935-4688558 [Escherichia coli O121:H19]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13789.59 Da        Isoelectric Point: 6.7387

>AT78985 WP_024174207.1 NZ_CP022407:c4689356-4688982 [Escherichia coli O121:H19]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS

Download         Length: 375 bp

>AT78985 NZ_CP022407:c4689356-4688982 [Escherichia coli O121:H19]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5Y7S6

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y7S1

References