Detailed information of TA system TA078984

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4637532..4638316
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: CGC46_RS24675
• Protein ID: WP_000854717.1
• Coordinates: 4637532..4637858 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CGC46_RS24680
• Protein ID: WP_001285487.1
• Coordinates: 4637948..4638316 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS24645	4632827..4633804	+	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-
CGC46_RS24650	4633801..4634979	+	1179	WP_000094965.1	type II secretion system protein GspL	-
CGC46_RS24655	4634981..4635517	+	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
CGC46_RS24660	4635860..4636702	-	843	WP_024174205.1	DUF4942 domain-containing protein	-
CGC46_RS24665	4636787..4636984	-	198	WP_000839243.1	DUF957 domain-containing protein	-
CGC46_RS24670	4636996..4637484	-	489	WP_000761730.1	hypothetical protein	-
CGC46_RS24675	4637532..4637858	-	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
CGC46_RS24680	4637948..4638316	-	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CGC46_RS24685	4638392..4638613	-	222	WP_000692312.1	DUF987 domain-containing protein	-
CGC46_RS24690	4638676..4639152	-	477	WP_001186728.1	RadC family protein	-
CGC46_RS24695	4639168..4639641	-	474	WP_000855068.1	antirestriction protein	-
CGC46_RS24700	4639735..4639980	-	246	WP_032212706.1	hypothetical protein	-
CGC46_RS24705	4639980..4640288	-	309	Protein_4520	DUF932 domain-containing protein	-
CGC46_RS24715	4641570..4641776	-	207	WP_000632607.1	AlpA family transcriptional regulator	-
CGC46_RS24720	4642267..4642440	+	174	Protein_4523	N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T78984 WP_000854717.1 NZ_CP022407:c4637858-4637532 [Escherichia coli O121:H19]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T78984 NZ_CP022407:c4637858-4637532 [Escherichia coli O121:H19]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT78984 WP_001285487.1 NZ_CP022407:c4638316-4637948 [Escherichia coli O121:H19]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT78984 NZ_CP022407:c4638316-4637948 [Escherichia coli O121:H19]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8