Detailed information of TA system TA078983

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4512696..4513533
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: CGC46_RS24010
• Protein ID: WP_000854687.1
• Coordinates: 4513153..4513533 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LJJ3
• Locus tag: CGC46_RS24005
• Protein ID: WP_001443392.1
• Coordinates: 4512696..4513064 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS23970	4507802..4508185	+	384	Protein_4384	DUF932 domain-containing protein	-
CGC46_RS23975	4508695..4510473	+	1779	WP_001243192.1	reverse transcriptase/maturase family protein	-
CGC46_RS23980	4510592..4511032	+	441	Protein_4386	DUF932 domain-containing protein	-
CGC46_RS23985	4511032..4511277	+	246	WP_001164974.1	hypothetical protein	-
CGC46_RS23990	4511371..4511844	+	474	WP_000855068.1	antirestriction protein	-
CGC46_RS23995	4511860..4512336	+	477	WP_001186728.1	RadC family protein	-
CGC46_RS24000	4512399..4512620	+	222	WP_000692312.1	DUF987 domain-containing protein	-
CGC46_RS24005	4512696..4513064	+	369	WP_001443392.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CGC46_RS24010	4513153..4513533	+	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
CGC46_RS24015	4513530..4514018	+	489	WP_001054228.1	hypothetical protein	-
CGC46_RS24020	4514038..4514235	+	198	WP_000772026.1	DUF957 domain-containing protein	-
CGC46_RS24025	4514320..4515165	+	846	WP_001274557.1	DUF4942 domain-containing protein	-
CGC46_RS24035	4515465..4515971	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
CGC46_RS24040	4516050..4517891	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T78983 WP_000854687.1 NZ_CP022407:4513153-4513533 [Escherichia coli O121:H19]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T78983 NZ_CP022407:4513153-4513533 [Escherichia coli O121:H19]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13781.54 Da
• Isoelectric Point: 6.2049

>AT78983 WP_001443392.1 NZ_CP022407:4512696-4513064 [Escherichia coli O121:H19]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT78983 NZ_CP022407:4512696-4513064 [Escherichia coli O121:H19]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67

Antitoxin

• AlphaFold DB: A0A7U9LJJ3