Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4051516..4052147 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name hicA Uniprot ID A0A377MSE5
Locus tag CGC46_RS21430 Protein ID WP_001259383.1
Coordinates 4051516..4051791 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag CGC46_RS21435 Protein ID WP_000593555.1
Coordinates 4051788..4052147 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS21415 4046520..4047587 + 1068 WP_000361470.1 HlyD family secretion protein -
CGC46_RS21420 4047584..4050319 + 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
CGC46_RS21425 4050319..4051443 + 1125 WP_001343434.1 ABC transporter permease -
CGC46_RS21430 4051516..4051791 + 276 WP_001259383.1 type II toxin-antitoxin system HicA family toxin Toxin
CGC46_RS21435 4051788..4052147 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CGC46_RS21440 4052267..4052668 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
CGC46_RS21445 4052674..4053480 - 807 WP_000173673.1 nickel import ATP-binding protein NikE -
CGC46_RS21450 4053477..4054241 - 765 WP_001136244.1 nickel import ATP-binding protein NikD -
CGC46_RS21455 4054241..4055074 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
CGC46_RS21460 4055071..4056015 - 945 WP_000947089.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4043491..4052147 8656


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10209.98 Da        Isoelectric Point: 10.7076

>T78980 WP_001259383.1 NZ_CP022407:4051516-4051791 [Escherichia coli O121:H19]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGPRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T78980 NZ_CP022407:4051516-4051791 [Escherichia coli O121:H19]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTCCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT78980 WP_000593555.1 NZ_CP022407:4051788-4052147 [Escherichia coli O121:H19]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT78980 NZ_CP022407:4051788-4052147 [Escherichia coli O121:H19]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MSE5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References