Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3256749..3257246 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CGC46_RS17385 Protein ID WP_001173343.1
Coordinates 3256749..3256922 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W5Y3H9
Locus tag CGC46_RS17390 Protein ID WP_071532590.1
Coordinates 3256950..3257246 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS17375 3253419..3254876 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
CGC46_RS17380 3255113..3256630 + 1518 WP_001295082.1 lysine--tRNA ligase -
CGC46_RS17385 3256749..3256922 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CGC46_RS17390 3256950..3257246 - 297 WP_071532590.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CGC46_RS17400 3257473..3257745 - 273 WP_000405647.1 N-acetyltransferase -
CGC46_RS17405 3257757..3257987 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
CGC46_RS17410 3258168..3259799 + 1632 WP_001216468.1 sensor histidine kinase -
CGC46_RS17415 3259796..3260515 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / nleB1 / nleE / efa1 3218483..3257246 38763


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T78977 WP_001173343.1 NZ_CP022407:c3256922-3256749 [Escherichia coli O121:H19]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T78977 NZ_CP022407:c3256922-3256749 [Escherichia coli O121:H19]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.04 Da        Isoelectric Point: 4.2931

>AT78977 WP_071532590.1 NZ_CP022407:c3257246-3256950 [Escherichia coli O121:H19]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT78977 NZ_CP022407:c3257246-3256950 [Escherichia coli O121:H19]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y3H9

References