Detailed information of TA system TA078975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2458017..2458854
• Replicon: chromosome
• Accession: NZ_CP022407
• Organism: Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: CGC46_RS13385
• Protein ID: WP_000227784.1
• Coordinates: 2458312..2458854 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: CGC46_RS13380
• Protein ID: WP_001297137.1
• Coordinates: 2458017..2458328 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGC46_RS13355	2453037..2453984	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
CGC46_RS13360	2454006..2455997	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
CGC46_RS13365	2455987..2456601	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
CGC46_RS13370	2456601..2456930	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
CGC46_RS13375	2456942..2457832	+	891	WP_000971336.1	heme o synthase	-
CGC46_RS13380	2458017..2458328	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
CGC46_RS13385	2458312..2458854	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
CGC46_RS13390	2458910..2459845	-	936	WP_001297127.1	sel1 repeat family protein	-
CGC46_RS13395	2460253..2461617	+	1365	WP_001000945.1	MFS transporter	-
CGC46_RS13400	2461745..2462236	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
CGC46_RS13405	2462404..2463315	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T78975 WP_000227784.1 NZ_CP022407:2458312-2458854 [Escherichia coli O121:H19]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T78975 NZ_CP022407:2458312-2458854 [Escherichia coli O121:H19]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT78975 WP_001297137.1 NZ_CP022407:2458017-2458328 [Escherichia coli O121:H19]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT78975 NZ_CP022407:2458017-2458328 [Escherichia coli O121:H19]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3