Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2423940..2424558 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CGC46_RS13210 Protein ID WP_001291435.1
Coordinates 2424340..2424558 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CGC46_RS13205 Protein ID WP_000344800.1
Coordinates 2423940..2424314 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS13195 2419029..2420222 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CGC46_RS13200 2420245..2423394 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CGC46_RS13205 2423940..2424314 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CGC46_RS13210 2424340..2424558 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CGC46_RS13215 2424729..2425280 + 552 WP_000102569.1 maltose O-acetyltransferase -
CGC46_RS13220 2425396..2425866 + 471 WP_000136192.1 YlaC family protein -
CGC46_RS13225 2426030..2427580 + 1551 WP_001370355.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CGC46_RS13230 2427622..2427975 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CGC46_RS13240 2428354..2428665 + 312 WP_000409911.1 MGMT family protein -
CGC46_RS13245 2428696..2429268 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78974 WP_001291435.1 NZ_CP022407:2424340-2424558 [Escherichia coli O121:H19]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T78974 NZ_CP022407:2424340-2424558 [Escherichia coli O121:H19]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT78974 WP_000344800.1 NZ_CP022407:2423940-2424314 [Escherichia coli O121:H19]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT78974 NZ_CP022407:2423940-2424314 [Escherichia coli O121:H19]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References