Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1626576..1627047 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag CGC46_RS08935 Protein ID WP_001303511.1
Coordinates 1626769..1627047 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag CGC46_RS08930 Protein ID WP_001302048.1
Coordinates 1626576..1626767 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS08890 1621625..1623181 + 1557 WP_001066419.1 IS66 family transposase -
CGC46_RS29460 1623185..1623343 - 159 WP_001370098.1 hypothetical protein -
CGC46_RS08895 1623345..1623563 - 219 WP_000209148.1 DUF4014 family protein -
CGC46_RS08900 1623596..1623808 - 213 WP_000935423.1 hypothetical protein -
CGC46_RS08905 1623914..1624336 - 423 WP_001151187.1 DUF977 family protein -
CGC46_RS08910 1624377..1625339 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
CGC46_RS08915 1625362..1625787 - 426 WP_000693944.1 Rha family transcriptional regulator -
CGC46_RS08920 1625784..1626086 - 303 WP_000172738.1 transcriptional regulator -
CGC46_RS08925 1626184..1626555 + 372 WP_001169687.1 hypothetical protein -
CGC46_RS08930 1626576..1626767 + 192 WP_001302048.1 hypothetical protein Antitoxin
CGC46_RS08935 1626769..1627047 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CGC46_RS08945 1627400..1627699 + 300 WP_001240334.1 hypothetical protein -
CGC46_RS08950 1627842..1628006 - 165 WP_001331716.1 hypothetical protein -
CGC46_RS08955 1628412..1628633 + 222 WP_000560212.1 cell division protein FtsZ -
CGC46_RS08960 1628757..1631228 + 2472 WP_000048560.1 exonuclease -
CGC46_RS08965 1631290..1631559 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1585088..1639395 54307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T78972 WP_001303511.1 NZ_CP022407:1626769-1627047 [Escherichia coli O121:H19]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T78972 NZ_CP022407:1626769-1627047 [Escherichia coli O121:H19]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT78972 WP_001302048.1 NZ_CP022407:1626576-1626767 [Escherichia coli O121:H19]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT78972 NZ_CP022407:1626576-1626767 [Escherichia coli O121:H19]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References