Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1147881..1148519 Replicon chromosome
Accession NZ_CP022407
Organism Escherichia coli O121:H19 strain 16-9255

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CGC46_RS06020 Protein ID WP_000813794.1
Coordinates 1148343..1148519 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CGC46_RS06015 Protein ID WP_001270286.1
Coordinates 1147881..1148297 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CGC46_RS05995 1143033..1143974 - 942 WP_001251313.1 ABC transporter permease -
CGC46_RS06000 1143975..1144988 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
CGC46_RS06005 1145006..1146151 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
CGC46_RS06010 1146396..1147802 - 1407 WP_000760614.1 PLP-dependent aminotransferase family protein -
CGC46_RS06015 1147881..1148297 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CGC46_RS06020 1148343..1148519 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CGC46_RS06025 1148741..1148971 + 231 WP_000494244.1 YncJ family protein -
CGC46_RS06030 1149063..1151024 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CGC46_RS06035 1151097..1151633 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
CGC46_RS06040 1151686..1152900 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T78971 WP_000813794.1 NZ_CP022407:c1148519-1148343 [Escherichia coli O121:H19]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T78971 NZ_CP022407:c1148519-1148343 [Escherichia coli O121:H19]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT78971 WP_001270286.1 NZ_CP022407:c1148297-1147881 [Escherichia coli O121:H19]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT78971 NZ_CP022407:c1148297-1147881 [Escherichia coli O121:H19]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCACCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References