Detailed information of TA system TA078929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 45981..46777
• Replicon: plasmid unnamed2
• Accession: NZ_CP022371
• Organism: Bosea sp. AS-1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: CE453_RS01270
• Protein ID: WP_089172951.1
• Coordinates: 46259..46777 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: CE453_RS01265
• Protein ID: WP_089173002.1
• Coordinates: 45981..46271 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE453_RS01240	41223..42395	-	1173	WP_089172949.1	site-specific integrase	-
CE453_RS01245	42403..43493	-	1091	Protein_45	hypothetical protein	-
CE453_RS28375	43607..43732	+	126	Protein_46	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CE453_RS01250	43806..44069	+	264	WP_089172950.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CE453_RS01260	44738..45912	+	1175	Protein_48	DUF1612 domain-containing protein	-
CE453_RS01265	45981..46271	+	291	WP_089173002.1	DUF1778 domain-containing protein	Antitoxin
CE453_RS01270	46259..46777	+	519	WP_089172951.1	GNAT family N-acetyltransferase	Toxin
CE453_RS01280	47104..47801	-	698	Protein_51	DUF4336 domain-containing protein	-
CE453_RS01285	48020..48292	-	273	WP_089173003.1	DUF4326 domain-containing protein	-
CE453_RS01290	48358..48711	-	354	WP_089173004.1	DUF736 domain-containing protein	-
CE453_RS01295	49337..49552	-	216	Protein_54	antirestriction protein ArdC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..112144	112144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (49-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-89)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18358.94 Da
• Isoelectric Point: 7.9059

>T78929 WP_089172951.1 NZ_CP022371:46259-46777 [Bosea sp. AS-1]
VAGLTAAAPLSPPEALREDHETDGFDSGEPTLDAWLGRRARANQVSGASRTYVVCRGQRVVGYYALASGGIDLDAAPGRF
RRNMPDPLPVVVLGRLAIARDEQGHGLGHAMLRDAASRVLQAAERVGVALLVVHALSERAKQFYLGCGFKESPLDAMTLM
VRVKDLGDARAQ

Nucleotide

• Length: 519 bp

>T78929 NZ_CP022371:46259-46777 [Bosea sp. AS-1]
GTGGCAGGGCTGACTGCAGCCGCGCCGCTCAGCCCTCCGGAGGCGCTGCGGGAGGACCACGAGACCGACGGGTTCGACAG
CGGCGAGCCGACACTCGACGCATGGTTGGGCCGGCGCGCACGAGCCAACCAAGTCAGTGGCGCCTCGCGAACCTACGTCG
TCTGCCGGGGGCAGCGGGTGGTCGGCTACTATGCGCTCGCCAGCGGAGGAATCGATCTCGACGCCGCGCCGGGCCGGTTT
CGGCGCAATATGCCCGACCCGCTTCCTGTTGTGGTCCTGGGCCGTCTCGCCATTGCGCGCGACGAGCAAGGGCACGGCCT
TGGCCACGCCATGCTTCGCGACGCCGCCTCGAGAGTGCTGCAGGCGGCCGAGCGCGTCGGCGTGGCGCTGCTCGTCGTGC
ATGCGCTCTCGGAGCGCGCGAAGCAGTTTTACCTCGGTTGCGGATTCAAGGAGTCGCCGCTCGACGCCATGACGCTCATG
GTGAGGGTCAAAGACCTCGGCGACGCTCGAGCGCAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10766.32 Da
• Isoelectric Point: 10.3074

>AT78929 WP_089173002.1 NZ_CP022371:45981-46271 [Bosea sp. AS-1]
MAQARKKDIKSRSLINLRVSPEDRRLIDRAATATGKNRSEFMLNAARFAAEEALLDKVLFRVDAKTYDGLTAYLDEPPSA
DASLRKLMQTPSPWQG

Nucleotide

• Length: 291 bp

>AT78929 NZ_CP022371:45981-46271 [Bosea sp. AS-1]
ATGGCGCAGGCACGCAAGAAGGACATCAAATCCCGTTCGCTGATCAACCTACGGGTCTCCCCGGAGGACCGGCGGTTGAT
CGACCGCGCCGCCACGGCGACCGGCAAGAACAGGTCCGAGTTCATGCTGAACGCCGCCCGGTTCGCCGCCGAGGAGGCGC
TGCTCGACAAAGTTCTGTTCCGTGTCGACGCCAAGACCTACGACGGGCTGACCGCATACCTCGATGAGCCACCGTCCGCT
GACGCCAGCCTGCGCAAGCTGATGCAGACGCCGTCGCCGTGGCAGGGCTGA