Detailed information of TA system TA078927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_26
• Location: 511167..511782
• Replicon: plasmid TSH58_p05
• Accession: NZ_CP022369
• Organism: Azospirillum sp. TSH58

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: TSH58p_RS32590
• Protein ID: WP_109067838.1
• Coordinates: 511167..511421 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: TSH58p_RS32595
• Protein ID: WP_109067839.1
• Coordinates: 511414..511782 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TSH58p_RS32570	506319..507218	-	900	WP_109067835.1	4-hydroxy-tetrahydrodipicolinate synthase	-
TSH58p_RS32575	507604..508734	-	1131	WP_109067961.1	alpha-hydroxy-acid oxidizing protein	-
TSH58p_RS32580	508991..510427	+	1437	WP_109067836.1	FAD-binding oxidoreductase	-
TSH58p_RS32585	510424..511116	+	693	WP_109067837.1	XRE family transcriptional regulator	-
TSH58p_RS32590	511167..511421	+	255	WP_109067838.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TSH58p_RS32595	511414..511782	+	369	WP_109067839.1	helix-turn-helix transcriptional regulator	Antitoxin
TSH58p_RS32600	511775..512662	-	888	WP_109067840.1	TonB family protein	-
TSH58p_RS32605	512709..514805	-	2097	WP_109067841.1	TonB-dependent receptor	-
TSH58p_RS32610	514854..516287	-	1434	WP_109067842.1	thiol oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pvdE / adeG	1..568757	568757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-80)

Antitoxin

• Cro/C1-type helix-turn-helix domain (67-121)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9499.12 Da
• Isoelectric Point: 10.5794

>T78927 WP_109067838.1 NZ_CP022369:511167-511421 [Azospirillum sp. TSH58]
MHTIRYTAAALKTLRRMPRNTADLIRLKLQEVAADPANGRNVKKLKGRDGYRLHVGDWRIVYDLESGVLVLIVIEVGPRG
GIYE

Nucleotide

• Length: 255 bp

>T78927 NZ_CP022369:511167-511421 [Azospirillum sp. TSH58]
ATGCACACGATCCGCTATACCGCGGCGGCCCTGAAGACGCTTCGCCGAATGCCGCGCAACACGGCGGACCTGATCCGCCT
GAAGCTGCAGGAGGTGGCCGCCGACCCGGCGAACGGGCGGAACGTAAAGAAGCTGAAGGGCCGCGACGGCTACCGGCTCC
ACGTCGGCGACTGGCGGATCGTTTATGACCTCGAATCCGGCGTGCTGGTGCTGATCGTCATCGAAGTCGGCCCGAGAGGA
GGCATCTATGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13206.86 Da
• Isoelectric Point: 4.4493

>AT78927 WP_109067839.1 NZ_CP022369:511414-511782 [Azospirillum sp. TSH58]
MSDPAQIIRDGNGNPVFAVLPIAEYERLLQAADEAIATRAFDVYKATQPETFPDDVAERLLSGEHPVKVFRDHRGMTQAQ
LAEAAGLKQAYVSQIEAGTRGGSVDVLKRIADALQVDLADLT

Nucleotide

• Length: 369 bp

>AT78927 NZ_CP022369:511414-511782 [Azospirillum sp. TSH58]
ATGAGTGATCCCGCGCAGATCATCCGGGATGGCAACGGCAATCCGGTCTTCGCGGTTCTTCCCATCGCCGAGTATGAACG
GTTGCTGCAGGCCGCCGACGAAGCAATCGCGACCCGCGCCTTCGACGTCTACAAGGCAACCCAGCCGGAGACATTCCCCG
ATGACGTGGCTGAGCGGCTGCTGAGCGGCGAACATCCGGTGAAGGTCTTCCGCGACCATCGCGGCATGACACAGGCCCAA
CTCGCGGAAGCCGCCGGGCTCAAGCAAGCCTATGTCTCGCAGATCGAGGCCGGCACGCGTGGTGGTTCGGTGGACGTGCT
CAAGCGGATCGCCGACGCTCTCCAGGTGGACCTCGCCGACCTCACCTGA