Detailed information of TA system TA078921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 114435..114979
• Replicon: plasmid pSHE-CTX-M
• Accession: NZ_CP022359
• Organism: Shewanella bicestrii strain JAB-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CF168_RS21640
• Protein ID: WP_089069034.1
• Coordinates: 114704..114979 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CF168_RS21635
• Protein ID: WP_089069033.1
• Coordinates: 114435..114704 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF168_RS21610	109480..110718	-	1239	WP_089069028.1	efflux RND transporter periplasmic adaptor subunit	-
CF168_RS21615	110720..111997	-	1278	WP_089069029.1	TolC family protein	-
CF168_RS21620	112045..112437	-	393	WP_089069030.1	hypothetical protein	-
CF168_RS21625	112529..112879	-	351	WP_089069031.1	DUF3147 family protein	-
CF168_RS21630	113177..114157	-	981	WP_089069032.1	DNA-binding protein	-
CF168_RS21635	114435..114704	+	270	WP_089069033.1	ribbon-helix-helix protein, CopG family	Antitoxin
CF168_RS21640	114704..114979	+	276	WP_089069034.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF168_RS21645	114982..116169	+	1188	WP_089069035.1	hypothetical protein	-
CF168_RS21650	116175..117113	+	939	WP_089069036.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / tet(A) / blaSHV-12 / aph(6)-Id / aph(3'')-Ib / sul2	-	1..193338	193338
	-	flank	IS/Tn	-	-	117217..120222	3005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-44)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10757.47 Da
• Isoelectric Point: 9.5571

>T78921 WP_089069034.1 NZ_CP022359:114704-114979 [Shewanella bicestrii]
MELKWTSKALSDIARLYEFLATDHPQAAARTVQSLTRAPTVLLINPQIGEQLFEFMPREVRRLLIGQYELRYELQGQTVY
LLRLWHTREAR

Nucleotide

• Length: 276 bp

>T78921 NZ_CP022359:114704-114979 [Shewanella bicestrii]
ATGGAGTTGAAATGGACCAGTAAGGCTCTGTCAGATATCGCGCGACTCTACGAATTTCTCGCTACGGATCACCCGCAGGC
TGCGGCCCGAACGGTGCAGAGTCTGACGAGAGCGCCAACGGTTTTGTTGATCAACCCACAGATCGGTGAGCAGCTGTTCG
AGTTTATGCCGCGAGAAGTCCGCCGCTTGTTGATTGGCCAGTATGAACTACGCTATGAGCTGCAGGGGCAAACTGTCTAC
CTGTTACGATTGTGGCACACTCGAGAAGCACGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9900.18 Da
• Isoelectric Point: 5.0760

>AT78921 WP_089069033.1 NZ_CP022359:114435-114704 [Shewanella bicestrii]
MTQTATKVLTAHIPQPLAERLDQAAGRLDRSRGWIMKQALSAWLSQEEERERLTREALADVDAGQVIDHQAVLAWAESLS
SDTPLPVPR

Nucleotide

• Length: 270 bp

>AT78921 NZ_CP022359:114435-114704 [Shewanella bicestrii]
ATGACACAAACGGCGACCAAAGTATTGACGGCGCACATTCCACAACCCTTGGCAGAGCGGCTTGATCAGGCCGCTGGCCG
GCTCGATCGTTCGCGTGGGTGGATCATGAAACAAGCCTTGTCGGCCTGGCTGTCGCAAGAAGAAGAACGTGAACGGCTGA
CACGGGAAGCCCTTGCCGATGTAGATGCAGGCCAGGTGATTGATCATCAGGCTGTGCTCGCCTGGGCAGAGAGTCTTAGC
AGCGACACGCCCTTGCCGGTGCCGCGCTGA