Detailed information of TA system TA078915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 114300..114859
• Replicon: chromosome
• Accession: NZ_CP022352
• Organism: Vibrio sp. 2521-89

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CEQ48_RS00830
• Protein ID: WP_089069892.1
• Coordinates: 114581..114859 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0F2I7R4
• Locus tag: CEQ48_RS00825
• Protein ID: WP_045569736.1
• Coordinates: 114300..114584 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ48_RS00770	109712..109949	+	238	Protein_128	RNA-binding protein	-
CEQ48_RS00785	110183..110364	-	182	Protein_129	DUF645 family protein	-
CEQ48_RS00790	110658..111296	-	639	WP_089069888.1	HD domain-containing protein	-
CEQ48_RS20015	111461..112126	-	666	WP_089069889.1	hypothetical protein	-
CEQ48_RS00800	112339..113052	-	714	WP_000123659.1	Fic family protein	-
CEQ48_RS00810	113280..113558	-	279	WP_176470729.1	DUF645 family protein	-
CEQ48_RS00815	113662..113772	-	111	WP_086017781.1	DUF3265 domain-containing protein	-
CEQ48_RS00825	114300..114584	+	285	WP_045569736.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CEQ48_RS00830	114581..114859	+	279	WP_089069892.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEQ48_RS00835	115019..115555	-	537	WP_089069893.1	sugar O-acetyltransferase	-
CEQ48_RS00840	115741..116175	-	435	WP_089069894.1	GNAT family N-acetyltransferase	-
CEQ48_RS00845	116352..116642	-	291	WP_032469738.1	YkgJ family cysteine cluster protein	-
CEQ48_RS00850	116850..117134	-	285	WP_000048765.1	antibiotic biosynthesis monooxygenase	-
CEQ48_RS20020	117367..117538	-	172	Protein_141	DUF645 family protein	-
CEQ48_RS00865	117973..118233	-	261	WP_198301128.1	DUF3709 domain-containing protein	-
CEQ48_RS19820	118227..118421	-	195	WP_157724677.1	DUF3709 domain-containing protein	-
CEQ48_RS19825	118538..118576	-	39	WP_082798268.1	hypothetical protein	-
CEQ48_RS19830	118595..118716	-	122	Protein_145	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	78239..171673	93434
	-	inside	Integron	-	-	85142..168415	83273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10852.59 Da
• Isoelectric Point: 4.6909

>T78915 WP_089069892.1 NZ_CP022352:114581-114859 [Vibrio sp. 2521-89]
MIFWEEASLNDREKIFEFLYDFNPAAAEKTDELIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPNE

Nucleotide

• Length: 279 bp

>T78915 NZ_CP022352:114581-114859 [Vibrio sp. 2521-89]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCCGC
TGAAAAAACGGACGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATGGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10931.28 Da
• Isoelectric Point: 8.0775

>AT78915 WP_045569736.1 NZ_CP022352:114300-114584 [Vibrio sp. 2521-89]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDTWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT78915 NZ_CP022352:114300-114584 [Vibrio sp. 2521-89]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGTCGTGAACTCACTGAACAATTAGCTGAGCAACAACGCAAGGCATTATCTCACGATACATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0F2I7R4