Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56729..57254 Replicon plasmid pK516_NDM1
Accession NZ_CP022350
Organism Klebsiella michiganensis strain K516

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag CF000_RS31205 Protein ID WP_008322213.1
Coordinates 56729..57034 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag CF000_RS31210 Protein ID WP_032934863.1
Coordinates 57036..57254 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF000_RS32105 51830..51982 + 153 Protein_55 IS5/IS1182 family transposase -
CF000_RS31175 52054..53174 + 1121 WP_089046468.1 IS3 family transposase -
CF000_RS31180 53203..53949 + 747 Protein_57 IS5 family transposase -
CF000_RS31190 54111..55433 + 1323 WP_016479957.1 GntP family transporter -
CF000_RS31195 55632..56423 - 792 WP_008322208.1 site-specific integrase -
CF000_RS31200 56413..56727 - 315 WP_008322211.1 hypothetical protein -
CF000_RS31205 56729..57034 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
CF000_RS31210 57036..57254 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CF000_RS31215 58116..58813 - 698 Protein_63 IS1 family transposase -
CF000_RS31225 59026..61608 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / rmtC / blaNDM-1 htpB 1..106140 106140
- inside IScluster/Tn - - 45705..69714 24009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T78913 WP_008322213.1 NZ_CP022350:c57034-56729 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T78913 NZ_CP022350:c57034-56729 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT78913 WP_032934863.1 NZ_CP022350:c57254-57036 [Klebsiella michiganensis]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT78913 NZ_CP022350:c57254-57036 [Klebsiella michiganensis]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References