Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56729..57254 Replicon plasmid pK516_NDM1
Accession NZ_CP022350
Organism Klebsiella michiganensis strain K516

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag CF000_RS31205 Protein ID WP_008322213.1
Coordinates 56729..57034 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag CF000_RS31210 Protein ID WP_032934863.1
Coordinates 57036..57254 (-) Length 73 a.a.

Genomic Context

CF000_RS32105CF000_RS31175CF000_RS31180CF000_RS31190CF000_RS31225CF000_RS31195CF000_RS31200CF000_RS31205CF000_RS31210CF000_RS31215
Location: 51830..51982 (153 bp)
Type: Others
Protein ID: Protein_55
Location: 52054..53174 (1121 bp)
Type: Others
Protein ID: WP_089046468.1
Location: 53203..53949 (747 bp)
Type: Others
Protein ID: Protein_57
Location: 54111..55433 (1323 bp)
Type: Others
Protein ID: WP_016479957.1
Location: 59026..61608 (2583 bp)
Type: Others
Protein ID: WP_016479955.1
Location: 55632..56423 (792 bp)
Type: Others
Protein ID: WP_008322208.1
Location: 56413..56727 (315 bp)
Type: Others
Protein ID: WP_008322211.1
Location: 56729..57034 (306 bp)
Type: Toxin
Protein ID: WP_008322213.1
Location: 57036..57254 (219 bp)
Type: Antitoxin
Protein ID: WP_032934863.1
Location: 58116..58813 (698 bp)
Type: Others
Protein ID: Protein_63
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
CF000_RS3210551830..51982+153Protein_55IS5/IS1182 family transposase-
CF000_RS3117552054..53174+1121WP_089046468.1IS3 family transposase-
CF000_RS3118053203..53949+747Protein_57IS5 family transposase-
CF000_RS3119054111..55433+1323WP_016479957.1GntP family transporter-
CF000_RS3119555632..56423-792WP_008322208.1site-specific integrase-
CF000_RS3120056413..56727-315WP_008322211.1hypothetical protein-
CF000_RS3120556729..57034-306WP_008322213.1type II toxin-antitoxin system toxin CcdBToxin
CF000_RS3121057036..57254-219WP_032934863.1type II toxin-antitoxin system antitoxin CcdAAntitoxin
CF000_RS3121558116..58813-698Protein_63IS1 family transposase-
CF000_RS3122559026..61608+2583WP_016479955.1EAL domain-containing protein-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideNon-Mobilizable plasmidsul1 / rmtC / blaNDM-1htpB1..106140106140
-insideIScluster/Tn--45705..6971424009


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T78913 WP_008322213.1 NZ_CP022350:c57034-56729 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T78913 NZ_CP022350:c57034-56729 [Klebsiella michiganensis]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT78913 WP_032934863.1 NZ_CP022350:c57254-57036 [Klebsiella michiganensis]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT78913 NZ_CP022350:c57254-57036 [Klebsiella michiganensis]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A6C0NE27

Antitoxin

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Source
ID
Structure
AlphaFold DBA0A6C0NE25

References