Detailed information of TA system TA078912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 47665..48468
• Replicon: plasmid pK516_NDM1
• Accession: NZ_CP022350
• Organism: Klebsiella michiganensis strain K516

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: W8E895
• Locus tag: CF000_RS31150
• Protein ID: WP_016479963.1
• Coordinates: 47665..48195 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W8E6Q5
• Locus tag: CF000_RS31155
• Protein ID: WP_022652312.1
• Coordinates: 48199..48468 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF000_RS31125	42880..43170	+	291	WP_004201172.1	co-chaperone GroES	-
CF000_RS31130	43226..44866	+	1641	WP_004201176.1	chaperonin GroEL	-
CF000_RS32095	45055..45231	+	177	Protein_47	IS91 family transposase	-
CF000_RS32100	45492..45611	+	120	Protein_48	IS30 family transposase	-
CF000_RS31135	45621..46370	+	750	Protein_49	IS3 family transposase	-
CF000_RS31140	46517..47533	-	1017	WP_087728544.1	IS5-like element IS5 family transposase	-
CF000_RS31150	47665..48195	-	531	WP_016479963.1	GNAT family N-acetyltransferase	Toxin
CF000_RS31155	48199..48468	-	270	WP_022652312.1	DUF1778 domain-containing protein	Antitoxin
CF000_RS31160	49324..50313	+	990	WP_022652311.1	RepB family plasmid replication initiator protein	-
CF000_RS31165	50609..51348	-	740	Protein_54	site-specific integrase	-
CF000_RS32105	51830..51982	+	153	Protein_55	IS5/IS1182 family transposase	-
CF000_RS31175	52054..53174	+	1121	WP_089046468.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / rmtC / blaNDM-1	htpB	1..106140	106140
	-	inside	IScluster/Tn	-	-	45705..69714	24009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20350.24 Da
• Isoelectric Point: 6.4907

>T78912 WP_016479963.1 NZ_CP022350:c48195-47665 [Klebsiella michiganensis]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Nucleotide

• Length: 531 bp

>T78912 NZ_CP022350:c48195-47665 [Klebsiella michiganensis]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10269.70 Da
• Isoelectric Point: 5.7252

>AT78912 WP_022652312.1 NZ_CP022350:c48468-48199 [Klebsiella michiganensis]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Nucleotide

• Length: 270 bp

>AT78912 NZ_CP022350:c48468-48199 [Klebsiella michiganensis]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A6C0L7Q2

Antitoxin

• AlphaFold DB: A0A2Z3XDS5