Detailed information of TA system TA078911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 72313..72848
• Replicon: plasmid pK516_KPC
• Accession: NZ_CP022349
• Organism: Klebsiella michiganensis strain K516

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: CF000_RS30500
• Protein ID: WP_032693963.1
• Coordinates: 72313..72600 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CF000_RS30505
• Protein ID: WP_032693964.1
• Coordinates: 72597..72848 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF000_RS30470	67903..68535	+	633	WP_080884176.1	ParA family protein	-
CF000_RS30475	68535..68909	+	375	WP_088903436.1	hypothetical protein	-
CF000_RS30480	69149..70123	+	975	WP_080884175.1	StbA family protein	-
CF000_RS30485	70127..70519	+	393	WP_080884174.1	plasmid partitioning/stability family protein	-
CF000_RS30490	70795..71295	-	501	WP_080884173.1	hypothetical protein	-
CF000_RS30495	72042..72230	-	189	WP_080884172.1	hypothetical protein	-
CF000_RS30500	72313..72600	-	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CF000_RS30505	72597..72848	-	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CF000_RS30510	73015..74289	-	1275	WP_080884171.1	Y-family DNA polymerase	-
CF000_RS30515	74289..74714	-	426	WP_032693966.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
CF000_RS30520	74924..75154	+	231	WP_032693967.1	hypothetical protein	-
CF000_RS30525	75589..75822	+	234	WP_080884170.1	hypothetical protein	-
CF000_RS30530	75819..76154	+	336	WP_080884169.1	hypothetical protein	-
CF000_RS30535	76538..77239	+	702	WP_080884168.1	DNA methylase	-
CF000_RS30540	77239..77460	+	222	WP_053390281.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..126877	126877

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T78911 WP_032693963.1 NZ_CP022349:c72600-72313 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T78911 NZ_CP022349:c72600-72313 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAGGAATGGCAAAAGCTGGATAAGGCCATTCAGCAACAGTTTGC
GAAGAAGCTGAAGAAGTGCTGTGACGAACCACATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGCGCGTCAGGCTTTCGTCTGGTCTATCAGGTAATAGATGATCAACTGATTATTGCTGTTGTGGCTGTCGGAAAG
CGTGAACGTAGTGACGTGTACAATCTGGCCAGCGAACGGATGAGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT78911 WP_032693964.1 NZ_CP022349:c72848-72597 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT78911 NZ_CP022349:c72848-72597 [Klebsiella michiganensis]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAATCAGCCCGCTTTTTACTGCATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAACCAGCGACTGCTTGACGTGGATCTGGAC
ACATATCTATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2