Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4986666..4987285 Replicon chromosome
Accession NZ_CP022348
Organism Klebsiella michiganensis strain K516

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag CF000_RS24255 Protein ID WP_004099646.1
Coordinates 4986666..4986884 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag CF000_RS24260 Protein ID WP_004129911.1
Coordinates 4986911..4987285 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF000_RS24225 4982711..4982974 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
CF000_RS24230 4982974..4983114 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
CF000_RS24235 4983111..4983809 - 699 WP_014228203.1 GNAT family N-acetyltransferase -
CF000_RS24240 4983911..4985365 + 1455 WP_014837606.1 PLP-dependent aminotransferase family protein -
CF000_RS24245 4985340..4985810 - 471 WP_014228205.1 YlaC family protein -
CF000_RS24250 4985937..4986503 - 567 WP_014837607.1 maltose O-acetyltransferase -
CF000_RS24255 4986666..4986884 - 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
CF000_RS24260 4986911..4987285 - 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
CF000_RS24265 4987765..4990911 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
CF000_RS24270 4990934..4992127 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T78910 WP_004099646.1 NZ_CP022348:c4986884-4986666 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T78910 NZ_CP022348:c4986884-4986666 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT78910 WP_004129911.1 NZ_CP022348:c4987285-4986911 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT78910 NZ_CP022348:c4987285-4986911 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References