Detailed information of TA system TA078908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4284025..4284601
• Replicon: chromosome
• Accession: NZ_CP022348
• Organism: Klebsiella michiganensis strain K516

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A1D8JNH2
• Locus tag: CF000_RS20915
• Protein ID: WP_042933975.1
• Coordinates: 4284025..4284312 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CF000_RS20920
• Protein ID: WP_014837314.1
• Coordinates: 4284299..4284601 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF000_RS20885	4279368..4279886	-	519	WP_014837307.1	hypothetical protein	-
CF000_RS20890	4279883..4280683	-	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
CF000_RS20895	4280829..4281413	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
CF000_RS20900	4281561..4282343	-	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
CF000_RS20905	4282431..4283329	+	899	Protein_3978	LysR family transcriptional regulator	-
CF000_RS20910	4283323..4283766	+	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
CF000_RS20915	4284025..4284312	+	288	WP_042933975.1	BrnT family toxin	Toxin
CF000_RS20920	4284299..4284601	+	303	WP_014837314.1	BrnA antitoxin family protein	Antitoxin
CF000_RS20925	4284722..4286065	-	1344	WP_004128506.1	APC family permease	-
CF000_RS20930	4286062..4286973	-	912	WP_014837315.1	acetamidase/formamidase family protein	-
CF000_RS20935	4287426..4287761	-	336	WP_014837316.1	endoribonuclease SymE	-
CF000_RS20940	4288112..4289515	-	1404	WP_089046410.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T78908 WP_042933975.1 NZ_CP022348:4284025-4284312 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T78908 NZ_CP022348:4284025-4284312 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11549.14 Da
• Isoelectric Point: 9.7962

>AT78908 WP_014837314.1 NZ_CP022348:4284299-4284601 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSGIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT78908 NZ_CP022348:4284299-4284601 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A1D8JNH2