Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4227603..4228407 Replicon chromosome
Accession NZ_CP022348
Organism Klebsiella michiganensis strain K516

Toxin (Protein)


Gene name yeeV Uniprot ID A0A486SQP3
Locus tag CF000_RS20650 Protein ID WP_040217122.1
Coordinates 4228018..4228407 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7H4M120
Locus tag CF000_RS20645 Protein ID WP_014837280.1
Coordinates 4227603..4227962 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CF000_RS20615 4224079..4224711 - 633 WP_071886307.1 inovirus Gp2 family protein -
CF000_RS20620 4225251..4226075 + 825 WP_040217125.1 DUF945 domain-containing protein -
CF000_RS20625 4226081..4226329 + 249 WP_044347584.1 DUF905 domain-containing protein -
CF000_RS20630 4226409..4226852 + 444 WP_014837277.1 antirestriction protein -
CF000_RS20635 4226864..4227343 + 480 WP_014837278.1 DNA repair protein RadC -
CF000_RS20640 4227357..4227578 + 222 WP_014837279.1 DUF987 domain-containing protein -
CF000_RS20645 4227603..4227962 + 360 WP_014837280.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CF000_RS20650 4228018..4228407 + 390 WP_040217122.1 TA system toxin CbtA family protein Toxin
CF000_RS20655 4228494..4229339 + 846 WP_014837282.1 DUF4942 domain-containing protein -
CF000_RS20660 4229481..4230278 + 798 WP_014837283.1 helix-turn-helix transcriptional regulator -
CF000_RS20665 4230456..4231199 - 744 WP_014837284.1 hypothetical protein -
CF000_RS20670 4231365..4231799 - 435 WP_004098173.1 VOC family protein -
CF000_RS20675 4231958..4232281 - 324 WP_014837285.1 endoribonuclease SymE -
CF000_RS20680 4232571..4233365 - 795 WP_042933970.1 DNA/RNA non-specific endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4206328..4232281 25953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(3-111)

Antitoxin

Antitoxin CbeA

(16-114)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14477.57 Da        Isoelectric Point: 8.9843

>T78907 WP_040217122.1 NZ_CP022348:4228018-4228407 [Klebsiella michiganensis]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITSIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Download         Length: 390 bp

>T78907 NZ_CP022348:4228018-4228407 [Klebsiella michiganensis]
ATGCAAACCAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTTACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACATCCATTGATATCCTCCGGGCCAGAAAGGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGTGCTCCAGGCAAGCGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13416.24 Da        Isoelectric Point: 7.0268

>AT78907 WP_014837280.1 NZ_CP022348:4227603-4227962 [Klebsiella michiganensis]
MSKKTTIATHDISESWWGLRRSVSACFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQSFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Download         Length: 360 bp

>AT78907 NZ_CP022348:4227603-4227962 [Klebsiella michiganensis]
TTGTCGAAGAAAACCACGATAGCAACGCATGATATCAGCGAATCCTGGTGGGGGCTCAGACGCAGTGTCTCCGCGTGTTT
TGGTGCCCGACTGGTCCAGGAGGGAAACCGCCTGCATTACCTGGCTGACCGCGCGAATTTTAACGGACAGTTCTGCGATG
CGGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACTGCGTCACGCTGTATGCAAAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486SQP3

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4M120

References