Detailed information of TA system TA078905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3455218..3455765
• Replicon: chromosome
• Accession: NZ_CP022348
• Organism: Klebsiella michiganensis strain K516

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CF000_RS16850
• Protein ID: WP_014839830.1
• Coordinates: 3455457..3455765 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0H3H0P7
• Locus tag: CF000_RS16845
• Protein ID: WP_014227298.1
• Coordinates: 3455218..3455454 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CF000_RS16830	3451425..3452591	+	1167	WP_014839828.1	multidrug effflux MFS transporter	-
CF000_RS16835	3452752..3453654	+	903	WP_009654080.1	N-acetylmuramic acid 6-phosphate etherase	-
CF000_RS16840	3453679..3455112	+	1434	WP_014839829.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
CF000_RS16845	3455218..3455454	+	237	WP_014227298.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CF000_RS16850	3455457..3455765	+	309	WP_014839830.1	CcdB family protein	Toxin
CF000_RS16855	3455916..3456818	+	903	WP_014227300.1	EamA family transporter	-
CF000_RS16860	3456822..3457079	-	258	WP_038422923.1	hypothetical protein	-
CF000_RS16865	3457079..3458044	-	966	WP_014839832.1	Rpn family recombination-promoting nuclease/putative transposase	-
CF000_RS16870	3458254..3458547	+	294	WP_014227302.1	hypothetical protein	-
CF000_RS16875	3458571..3459512	-	942	WP_014227303.1	LacI family transcriptional regulator	-
CF000_RS16880	3459807..3460118	+	312	WP_014839834.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11724.63 Da
• Isoelectric Point: 8.8012

>T78905 WP_014839830.1 NZ_CP022348:3455457-3455765 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGKE
VCNLNHQREVVKASMDFLFDGI

Nucleotide

• Length: 309 bp

>T78905 NZ_CP022348:3455457-3455765 [Klebsiella michiganensis]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGCCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTAAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8783.92 Da
• Isoelectric Point: 5.7928

>AT78905 WP_014227298.1 NZ_CP022348:3455218-3455454 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT78905 NZ_CP022348:3455218-3455454 [Klebsiella michiganensis]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H0P7