Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4636607..4637249 Replicon chromosome
Accession NZ_CP022345
Organism Bacillus thuringiensis strain c25

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag CCX84_RS24650 Protein ID WP_000635965.1
Coordinates 4636607..4636957 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag CCX84_RS24655 Protein ID WP_000004570.1
Coordinates 4636962..4637249 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCX84_RS24635 4633543..4634001 - 459 WP_000344242.1 SprT family protein -
CCX84_RS24640 4634197..4634313 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
CCX84_RS24645 4634371..4636539 - 2169 WP_061140076.1 RNA-binding transcriptional accessory protein -
CCX84_RS24650 4636607..4636957 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CCX84_RS24655 4636962..4637249 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
CCX84_RS24660 4637558..4638727 - 1170 WP_000390617.1 alanine racemase -
CCX84_RS24665 4638845..4639795 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
CCX84_RS24670 4639952..4640311 - 360 WP_000583417.1 holo-ACP synthase -
CCX84_RS24675 4640405..4640977 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
CCX84_RS24680 4640970..4641932 - 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T78895 WP_000635965.1 NZ_CP022345:c4636957-4636607 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T78895 NZ_CP022345:c4636957-4636607 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT78895 WP_000004570.1 NZ_CP022345:c4637249-4636962 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT78895 NZ_CP022345:c4637249-4636962 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References