Detailed information of TA system TA078894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2534408..2535386
• Replicon: chromosome
• Accession: NZ_CP022345
• Organism: Bacillus thuringiensis strain c25

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: CCX84_RS13495
• Protein ID: WP_000624979.1
• Coordinates: 2534649..2535386 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: CCX84_RS13490
• Protein ID: WP_000237818.1
• Coordinates: 2534408..2534536 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCX84_RS13460	2529895..2530146	+	252	WP_000827561.1	response regulator transcription factor	-
CCX84_RS13465	2530387..2530989	-	603	WP_000517260.1	hypothetical protein	-
CCX84_RS13470	2531015..2531824	-	810	WP_000664542.1	C39 family peptidase	-
CCX84_RS13475	2532069..2533538	-	1470	WP_000287533.1	beta-Ala-His dipeptidase	-
CCX84_RS13480	2533750..2534139	+	390	WP_000713864.1	YxeA family protein	-
CCX84_RS13485	2534159..2534335	-	177	WP_000852627.1	stage II sporulation protein SB	-
CCX84_RS13490	2534408..2534536	-	129	WP_000237818.1	hypothetical protein	Antitoxin
CCX84_RS13495	2534649..2535386	-	738	WP_000624979.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CCX84_RS13505	2535624..2536361	-	738	WP_000594151.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CCX84_RS13510	2536528..2537010	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
CCX84_RS13515	2537118..2538803	+	1686	WP_061139288.1	alpha-keto acid decarboxylase family protein	-
CCX84_RS13520	2538961..2539743	-	783	WP_000381800.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28311.04 Da
• Isoelectric Point: 8.2998

>T78894 WP_000624979.1 NZ_CP022345:c2535386-2534649 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHMEQLNEQKAVQVENVGVIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T78894 NZ_CP022345:c2535386-2534649 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
AGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTAGATATTGCATTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATACGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATGGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGGTTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT78894 WP_000237818.1 NZ_CP022345:c2534536-2534408 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT78894 NZ_CP022345:c2534536-2534408 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAACAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5