Detailed information of TA system TA078893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1250178..1251095
• Replicon: chromosome
• Accession: NZ_CP022341
• Organism: Bacillus velezensis strain 157

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: CFN60_RS06575
• Protein ID: WP_007407256.1
• Coordinates: 1250349..1251095 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: CFN60_RS06570
• Protein ID: WP_003154807.1
• Coordinates: 1250178..1250348 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFN60_RS06535	1245411..1247033	+	1623	WP_022553476.1	pyocin knob domain-containing protein	-
CFN60_RS06540	1247046..1247417	+	372	WP_022553475.1	hypothetical protein	-
CFN60_RS06545	1247422..1247619	+	198	WP_007610833.1	XkdX family protein	-
CFN60_RS06550	1247676..1248437	+	762	WP_022553474.1	hypothetical protein	-
CFN60_RS06555	1248489..1248752	+	264	WP_022553473.1	hemolysin XhlA family protein	-
CFN60_RS06560	1248766..1249029	+	264	WP_003154813.1	phage holin	-
CFN60_RS06565	1249043..1249921	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
CFN60_RS06570	1250178..1250348	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
CFN60_RS06575	1250349..1251095	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CFN60_RS06580	1251200..1252198	-	999	WP_003154805.1	inorganic phosphate transporter	-
CFN60_RS06585	1252211..1252828	-	618	WP_003154804.1	DUF47 domain-containing protein	-
CFN60_RS06590	1253114..1254430	-	1317	WP_007610842.1	amino acid permease	-
CFN60_RS06595	1254752..1255702	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T78893 WP_007407256.1 NZ_CP022341:c1251095-1250349 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T78893 NZ_CP022341:c1251095-1250349 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCGGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGAAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACTGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT78893 WP_003154807.1 NZ_CP022341:c1250348-1250178 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT78893 NZ_CP022341:c1250348-1250178 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAGA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14