Detailed information of TA system TA078892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 485980..486617
• Replicon: chromosome
• Accession: NZ_CP022341
• Organism: Bacillus velezensis strain 157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: CFN60_RS02470
• Protein ID: WP_003156187.1
• Coordinates: 486267..486617 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: CFN60_RS02465
• Protein ID: WP_003156188.1
• Coordinates: 485980..486261 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFN60_RS02445	482345..482944	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
CFN60_RS02450	483037..483402	+	366	WP_022552632.1	holo-ACP synthase	-
CFN60_RS02455	483567..484574	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
CFN60_RS02460	484691..485860	+	1170	WP_022552633.1	alanine racemase	-
CFN60_RS02465	485980..486261	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
CFN60_RS02470	486267..486617	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CFN60_RS02475	486735..487556	+	822	WP_003156182.1	STAS domain-containing protein	-
CFN60_RS02480	487561..487926	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
CFN60_RS02485	487929..488330	+	402	WP_003156178.1	anti-sigma regulatory factor	-
CFN60_RS02490	488342..489349	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
CFN60_RS02495	489413..489742	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
CFN60_RS02500	489739..490221	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
CFN60_RS02505	490187..490975	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
CFN60_RS02510	490975..491577	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T78892 WP_003156187.1 NZ_CP022341:486267-486617 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T78892 NZ_CP022341:486267-486617 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT78892 WP_003156188.1 NZ_CP022341:485980-486261 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT78892 NZ_CP022341:485980-486261 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5