Detailed information of TA system TA078890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2644537..2645146
• Replicon: chromosome
• Accession: NZ_CP022340
• Organism: Enterococcus mundtii strain EMB156

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CO646_RS12610
• Protein ID: WP_010734231.1
• Coordinates: 2644958..2645146 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V5XTD1
• Locus tag: CO646_RS12605
• Protein ID: WP_010734232.1
• Coordinates: 2644537..2644923 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO646_RS12585	2640311..2640946	-	636	WP_010734236.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
CO646_RS12590	2641078..2641812	+	735	WP_096081803.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
CO646_RS12595	2641802..2642089	+	288	WP_010734234.1	GIY-YIG nuclease family protein	-
CO646_RS12600	2642211..2644142	-	1932	WP_010734233.1	acetyltransferase	-
CO646_RS12605	2644537..2644923	-	387	WP_010734232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CO646_RS12610	2644958..2645146	-	189	WP_010734231.1	type II toxin-antitoxin system HicA family toxin	Toxin
CO646_RS14420	2646672..2646818	-	147	WP_155237725.1	hypothetical protein	-
CO646_RS12625	2647068..2647943	-	876	WP_010734230.1	YitT family protein	-
CO646_RS12630	2648151..2649917	-	1767	WP_010734229.1	aspartate--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2632678..2647943	15265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-62)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-101)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6983.18 Da
• Isoelectric Point: 11.0565

>T78890 WP_010734231.1 NZ_CP022340:c2645146-2644958 [Enterococcus mundtii]
MPMTIREAEKLLKDAGFVEVKGGKGSHRKFTKKDHPRPVILTSHSKELSRRVEDSVRKAVGI

Nucleotide

• Length: 189 bp

>T78890 NZ_CP022340:c2645146-2644958 [Enterococcus mundtii]
ATGCCCATGACAATCAGAGAAGCAGAAAAGCTACTGAAAGATGCTGGCTTTGTTGAAGTGAAAGGTGGGAAGGGCTCACA
TAGGAAATTCACTAAAAAGGATCATCCTAGACCTGTTATCCTTACTAGCCATTCAAAAGAGTTATCTAGAAGAGTAGAAG
ATTCTGTTCGAAAAGCAGTGGGCATTTAA

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.41 Da
• Isoelectric Point: 4.4851

>AT78890 WP_010734232.1 NZ_CP022340:c2644923-2644537 [Enterococcus mundtii]
MKKVYPAIFEKDPVGYGIYFPDIEGAVTQSKDIIEGLEVASDALGIMLGDLVENNKPLPKPTNINDLSIDSEREFATLIS
VDLNDYLKDVQLDKKTIKIPHWLNVRATNEGINFSKTMSEALVQKLNI

Nucleotide

• Length: 387 bp

>AT78890 NZ_CP022340:c2644923-2644537 [Enterococcus mundtii]
ATGAAAAAAGTTTATCCAGCTATTTTTGAAAAGGATCCTGTTGGATATGGAATTTATTTTCCTGATATCGAAGGAGCAGT
GACACAATCAAAAGATATTATCGAGGGGTTAGAAGTTGCATCGGATGCTTTAGGGATCATGCTCGGTGATTTAGTTGAGA
ATAATAAACCTTTGCCAAAGCCAACTAATATCAATGATCTTTCGATTGATTCTGAGCGTGAATTTGCTACTTTGATTTCT
GTGGATCTAAATGATTACTTAAAAGATGTCCAACTAGATAAAAAGACAATAAAAATTCCTCATTGGTTAAATGTGCGCGC
AACAAATGAAGGAATAAATTTTTCAAAAACAATGTCTGAAGCTTTGGTTCAAAAATTAAATATATGA

Structures

Antitoxin

• AlphaFold DB: V5XTD1