Detailed information of TA system TA078887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3441740..3442358
• Replicon: chromosome
• Accession: NZ_CP022338
• Organism: Yersinia enterocolitica strain FORC066

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: FORC066_RS16095
• Protein ID: WP_002208622.1
• Coordinates: 3442155..3442358 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: Q6TU35
• Locus tag: FORC066_RS16090
• Protein ID: WP_005167670.1
• Coordinates: 3441740..3442108 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC066_RS16055 (FORC066_3418)	3437403..3437663	+	261	WP_050164417.1	type B 50S ribosomal protein L31	-
FORC066_RS16060 (FORC066_3419)	3437679..3437822	+	144	WP_002208618.1	type B 50S ribosomal protein L36	-
FORC066_RS16065 (FORC066_3420)	3437950..3438828	-	879	WP_050164414.1	metal ABC transporter substrate-binding protein	-
FORC066_RS16070 (FORC066_3421)	3438877..3439734	-	858	WP_050164412.1	metal ABC transporter permease	-
FORC066_RS16075 (FORC066_3422)	3439731..3440459	-	729	WP_019081288.1	ABC transporter ATP-binding protein	-
FORC066_RS16080 (FORC066_3423)	3440456..3440596	-	141	WP_172653924.1	hypothetical protein	-
FORC066_RS16085 (FORC066_3424)	3441229..3441582	+	354	WP_019082548.1	hypothetical protein	-
FORC066_RS16090 (FORC066_3425)	3441740..3442108	+	369	WP_005167670.1	Hha toxicity modulator TomB	Antitoxin
FORC066_RS16095 (FORC066_3426)	3442155..3442358	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
FORC066_RS16105 (FORC066_3427)	3443204..3443509	+	306	WP_032901164.1	MGMT family protein	-
FORC066_RS16110 (FORC066_3428)	3443561..3444091	-	531	WP_050160272.1	YbaY family lipoprotein	-
FORC066_RS16115 (FORC066_3430)	3444349..3445212	+	864	WP_019082546.1	acyl-CoA thioesterase II	-
FORC066_RS16120 (FORC066_3431)	3445432..3446721	-	1290	WP_005167660.1	ammonium transporter AmtB	-
FORC066_RS16125 (FORC066_3432)	3446761..3447099	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T78887 WP_002208622.1 NZ_CP022338:3442155-3442358 [Yersinia enterocolitica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T78887 NZ_CP022338:3442155-3442358 [Yersinia enterocolitica]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACATTGGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGACGATGAGCTGGAATTGTTTTACTCAGCAGCAGACCACCGCTTAGCTGAACTCACAATGAATAAAC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14134.86 Da
• Isoelectric Point: 4.4085

>AT78887 WP_005167670.1 NZ_CP022338:3441740-3442108 [Yersinia enterocolitica]
MDEYSPKRHDVAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGGDLSELVEEYL
DDTYTLFSSYGINDPELQRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT78887 NZ_CP022338:3441740-3442108 [Yersinia enterocolitica]
ATGGATGAGTACTCACCTAAGCGGCATGACGTTGCCCAATTGAAGTTCCTGTGCGAGAGTTTGTATGATGAAGGCATTGC
TACCCTTGGTGATAGCCATCATGGCTGGGTAAATGATCCAACTTCAGCCGTAAACCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGCGGCGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTACGGAATCAATGATCCGGAATTGCAGCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: A0A8B6L1C1