Detailed information of TA system TA078863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 270430..271018
• Replicon: chromosome
• Accession: NZ_CP022298
• Organism: Acinetobacter johnsonii strain IC001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A2W5RB36
• Locus tag: CFH90_RS01275
• Protein ID: WP_039890600.1
• Coordinates: 270430..270717 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CFH90_RS01280
• Protein ID: WP_126034950.1
• Coordinates: 270704..271018 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFH90_RS01255	265832..267460	-	1629	WP_039890603.1	iron ABC transporter permease	-
CFH90_RS01260	267542..268558	-	1017	WP_005401415.1	extracellular solute-binding protein	-
CFH90_RS01265	268580..269704	-	1125	WP_126034948.1	ABC transporter ATP-binding protein	-
CFH90_RS01275	270430..270717	+	288	WP_039890600.1	BrnT family toxin	Toxin
CFH90_RS01280	270704..271018	+	315	WP_126034950.1	BrnA antitoxin family protein	Antitoxin
CFH90_RS01285	271083..271709	-	627	WP_126034952.1	hypothetical protein	-
CFH90_RS01290	271696..272160	-	465	WP_126034954.1	hypothetical protein	-
CFH90_RS01295	272157..272558	-	402	Protein_245	hypothetical protein	-
CFH90_RS01300	272610..273829	+	1220	WP_126034956.1	IS3 family transposase	-
CFH90_RS01305	273930..274970	-	1041	WP_126034731.1	IS481 family transposase	-
CFH90_RS01310	275075..275587	-	513	Protein_248	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	261688..313785	52097
	-	inside	IScluster/Tn	-	katA	272610..285198	12588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11317.74 Da
• Isoelectric Point: 6.0889

>T78863 WP_039890600.1 NZ_CP022298:270430-270717 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKKQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T78863 NZ_CP022298:270430-270717 [Acinetobacter johnsonii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAAACAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTGTATTTGAAGATCCTCTGCGGATCTCAATCCAAGACAGACATACCAATGGCGAAGAACGTTGGCAAACCATTGGTA
GGGTAAAAGGCGTACTAATGCTGTTAGTTGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATTATTAGT
GCAAGACAAGTCACTAAAGCGGAGCGAAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12233.90 Da
• Isoelectric Point: 10.0927

>AT78863 WP_126034950.1 NZ_CP022298:270704-271018 [Acinetobacter johnsonii]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDDQLDLSDIPEITDWSNAVRHGQFYRPVKQQTSVRLDADVLAWLKSQ
GKGYQTRMNKILREAMLNERKNHR

Nucleotide

• Length: 315 bp

>AT78863 NZ_CP022298:270704-271018 [Acinetobacter johnsonii]
ATGAGCATGGTTAGATATACACGCAAAGAGCTGAATGAAAACTTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCTAAGGGAACTGTGCCTGATGATCAATTAGACCTATCTGATATTCCAGAAATAACCGACTGGAGTAATGCAGTTCGTC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAGACTTCAGTTCGTCTAGATGCTGATGTACTAGCATGGTTGAAATCGCAA
GGTAAAGGCTATCAAACACGTATGAACAAAATTCTACGTGAAGCAATGCTAAATGAACGTAAAAATCATCGATAA

Structures

Toxin

• AlphaFold DB: A0A2W5RB36