Detailed information of TA system TA078847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2095074..2095588
• Replicon: chromosome
• Accession: NZ_CP022291
• Organism: Staphylococcus aureus strain EDCC5464

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: CFN13_RS10660
• Protein ID: WP_000581792.1
• Coordinates: 2095074..2095283 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: CFN13_RS10665
• Protein ID: WP_000138928.1
• Coordinates: 2095295..2095588 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFN13_RS10640	2090582..2091940	-	1359	WP_000611469.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
CFN13_RS10645	2091955..2093025	-	1071	WP_000159632.1	D-alanine--D-alanine ligase	-
CFN13_RS10650	2093343..2094545	+	1203	WP_001109944.1	rod shape-determining protein RodA	-
CFN13_RS10655	2094809..2094946	-	138	WP_000828354.1	hypothetical protein	-
CFN13_RS10660	2095074..2095283	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
CFN13_RS10665	2095295..2095588	-	294	WP_000138928.1	copper-sensing transcriptional repressor CsoR	Antitoxin
CFN13_RS10670	2095754..2097238	+	1485	WP_100185121.1	cardiolipin synthase	-
CFN13_RS10675	2097266..2097913	+	648	WP_001187617.1	HD domain-containing protein	-
CFN13_RS10685	2098391..2099263	-	873	WP_000725802.1	membrane protein insertase YidC	-
CFN13_RS10690	2099349..2099990	-	642	WP_000483153.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T78847 WP_000581792.1 NZ_CP022291:c2095283-2095074 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T78847 NZ_CP022291:c2095283-2095074 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11184.82 Da
• Isoelectric Point: 6.2243

>AT78847 WP_000138928.1 NZ_CP022291:c2095588-2095295 [Staphylococcus aureus]
MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT78847 NZ_CP022291:c2095588-2095295 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1