78821

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509820..510456 Replicon chromosome
Accession NZ_CP022287
Organism Bacillus subtilis strain SX01705

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BSSX_RS02605 Protein ID WP_003156187.1
Coordinates 510106..510456 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BSSX_RS02600 Protein ID WP_003225183.1
Coordinates 509820..510101 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSSX_RS02580 506179..506778 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
BSSX_RS02585 506873..507238 + 366 WP_015252768.1 holo-ACP synthase -
BSSX_RS02590 507404..508420 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
BSSX_RS02595 508535..509704 + 1170 WP_015382833.1 alanine racemase -
BSSX_RS02600 509820..510101 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BSSX_RS02605 510106..510456 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BSSX_RS02610 510572..511396 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BSSX_RS02615 511401..511766 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BSSX_RS02620 511770..512171 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
BSSX_RS02625 512183..513190 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
BSSX_RS02630 513252..513581 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
BSSX_RS02635 513578..514060 + 483 WP_003234299.1 anti-sigma B factor RsbW -
BSSX_RS02640 514026..514814 + 789 WP_015482833.1 RNA polymerase sigma factor SigB -
BSSX_RS02645 514814..515413 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T78821 WP_003156187.1 NZ_CP022287:510106-510456 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T78821 NZ_CP022287:510106-510456 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT78821 WP_003225183.1 NZ_CP022287:509820-510101 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT78821 NZ_CP022287:509820-510101 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References