Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 48250..48772 Replicon plasmid pSTEC299_2
Accession NZ_CP022281
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag CFI09_RS27045 Protein ID WP_000220560.1
Coordinates 48250..48531 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag CFI09_RS27050 Protein ID WP_000121743.1
Coordinates 48521..48772 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS26995 44097..45263 - 1167 WP_158639749.1 relaxase/mobilization nuclease domain-containing protein -
CFI09_RS27000 45266..45811 - 546 WP_001426313.1 hypothetical protein -
CFI09_RS27725 45806..45880 - 75 Protein_45 DNA distortion polypeptide 3 -
CFI09_RS27010 46136..46408 - 273 WP_000160399.1 hypothetical protein -
CFI09_RS27020 46640..46819 - 180 WP_000439078.1 hypothetical protein -
CFI09_RS27025 46863..47193 - 331 Protein_48 hypothetical protein -
CFI09_RS27030 47286..47501 - 216 WP_001180116.1 hypothetical protein -
CFI09_RS27035 47491..47736 - 246 WP_000356542.1 hypothetical protein -
CFI09_RS27040 47781..48104 - 324 WP_001181902.1 hypothetical protein -
CFI09_RS27045 48250..48531 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFI09_RS27050 48521..48772 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
CFI09_RS27055 49048..50067 - 1020 WP_101974025.1 IS110 family transposase -
CFI09_RS27060 51275..52144 + 870 WP_032347905.1 replication initiation protein -
CFI09_RS27065 52149..53000 + 852 WP_101974026.1 replication initiation protein -
CFI09_RS27070 53004..53447 + 444 Protein_57 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pic 1..59190 59190
- flank IS/Tn - - 49048..50067 1019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T78816 WP_000220560.1 NZ_CP022281:c48531-48250 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T78816 NZ_CP022281:c48531-48250 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT78816 WP_000121743.1 NZ_CP022281:c48772-48521 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT78816 NZ_CP022281:c48772-48521 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References