Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 119715..120250 Replicon plasmid pSTEC299_1
Accession NZ_CP022280
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag CFI09_RS26405 Protein ID WP_001270417.1
Coordinates 119963..120250 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag CFI09_RS26400 Protein ID WP_001132895.1
Coordinates 119715..119966 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS27710 114739..115050 - 312 Protein_120 hypothetical protein -
CFI09_RS27450 115184..115474 - 291 WP_199769559.1 DUF1281 domain-containing protein -
CFI09_RS27455 115471..115614 - 144 WP_001358230.1 DUF1281 domain-containing protein -
CFI09_RS26370 115779..116556 - 778 Protein_123 IS4 family transposase -
CFI09_RS26380 116792..117754 - 963 WP_101973954.1 ParB/RepB/Spo0J family partition protein -
CFI09_RS26385 117751..118956 - 1206 WP_000817644.1 AAA family ATPase -
CFI09_RS26395 119330..119542 + 213 WP_032334873.1 hypothetical protein -
CFI09_RS26400 119715..119966 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CFI09_RS26405 119963..120250 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFI09_RS26410 120249..120530 + 282 Protein_129 integrase core domain-containing protein -
CFI09_RS26420 121180..122156 + 977 Protein_130 RepB family plasmid replication initiator protein -
CFI09_RS26425 122416..123644 + 1229 WP_158639709.1 IS3 family transposase -
CFI09_RS26430 123721..124461 - 741 WP_101973955.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - paa / afaC-VII / exeG / stcE 1..191691 191691
- inside IScluster/Tn - - 94785..123644 28859


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T78815 WP_001270417.1 NZ_CP022280:119963-120250 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T78815 NZ_CP022280:119963-120250 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCTTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT78815 WP_001132895.1 NZ_CP022280:119715-119966 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT78815 NZ_CP022280:119715-119966 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References