Detailed information of TA system TA078814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 103424..104031
• Replicon: plasmid pSTEC299_1
• Accession: NZ_CP022280
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: CFI09_RS26250
• Protein ID: WP_101973941.1
• Coordinates: 103424..103810 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B7LIH9
• Locus tag: CFI09_RS26255
• Protein ID: WP_000493532.1
• Coordinates: 103807..104031 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS26205	98450..98656	+	207	WP_101973938.1	hemolysin expression modulator Hha	-
CFI09_RS26210	98731..99237	+	507	WP_101973988.1	IS66 family insertion sequence hypothetical protein	-
CFI09_RS26215	99465..99839	-	375	Protein_95	transposase	-
CFI09_RS26220	99926..100573	+	648	WP_101973939.1	IS66-like element accessory protein TnpA	-
CFI09_RS26225	100573..100884	+	312	WP_032211263.1	IS66 family insertion sequence element accessory protein TnpB	-
CFI09_RS26230	101482..101730	+	249	WP_199769555.1	reverse transcriptase N-terminal domain-containing protein	-
CFI09_RS26235	101793..101942	-	150	Protein_99	Hin recombinase	-
CFI09_RS26240	101942..102601	-	660	Protein_100	transposase zinc-binding domain-containing protein	-
CFI09_RS26245	102601..102966	-	366	WP_101973940.1	transposase	-
CFI09_RS26250	103424..103810	-	387	WP_101973941.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CFI09_RS26255	103807..104031	-	225	WP_000493532.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CFI09_RS26270	104758..105738	+	981	WP_101973943.1	plasmid segregation protein ParM	-
CFI09_RS26275	105741..106163	+	423	WP_000774832.1	plasmid partitioning/stability family protein	-
CFI09_RS26280	106167..106478	+	312	WP_101973944.1	hypothetical protein	-
CFI09_RS26285	106562..107281	+	720	WP_101973945.1	DUF1281 domain-containing protein	-
CFI09_RS26290	107297..108079	-	783	WP_101973946.1	IS21-like element ISSen3 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	paa / afaC-VII / exeG / stcE	1..191691	191691
	-	inside	IScluster/Tn	-	-	94785..123644	28859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (5-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14448.69 Da
• Isoelectric Point: 7.0732

>T78814 WP_101973941.1 NZ_CP022280:c103810-103424 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYESVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QFFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Nucleotide

• Length: 387 bp

>T78814 NZ_CP022280:c103810-103424 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAAGTGTAACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGTTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8361.39 Da
• Isoelectric Point: 5.6713

>AT78814 WP_000493532.1 NZ_CP022280:c104031-103807 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Nucleotide

• Length: 225 bp

>AT78814 NZ_CP022280:c104031-103807 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCTGT
TGAGATTCAGCGCCGTAAAGGTGGCGCGGCTGTACTAGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A086V9Y8