Detailed information of TA system TA078813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 35941..36698
• Replicon: plasmid pSTEC299_1
• Accession: NZ_CP022280
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: CFI09_RS25925
• Protein ID: WP_101973914.1
• Coordinates: 36213..36698 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: CFI09_RS25920
• Protein ID: WP_101973913.1
• Coordinates: 35941..36225 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS25870	31290..31499	+	210	WP_101973908.1	hemolysin expression modulator Hha	-
CFI09_RS25875	31537..32127	+	591	WP_101973907.1	DUF2726 domain-containing protein	-
CFI09_RS25880	32282..32746	+	465	WP_101973911.1	hypothetical protein	-
-	32898..32954	-	57	NuclAT_0	-	-
-	32898..32954	-	57	NuclAT_0	-	-
-	32898..32954	-	57	NuclAT_0	-	-
-	32898..32954	-	57	NuclAT_0	-	-
CFI09_RS25890	33002..33151	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
CFI09_RS25895	33435..33689	+	255	WP_000083830.1	replication regulatory protein RepA	-
CFI09_RS25905	33926..34000	+	75	WP_032144212.1	RepA leader peptide Tap	-
CFI09_RS25910	33993..35015	+	1023	WP_101973912.1	replication initiation protein	-
CFI09_RS25920	35941..36225	+	285	WP_101973913.1	DUF1778 domain-containing protein	Antitoxin
CFI09_RS25925	36213..36698	+	486	WP_101973914.1	GNAT family N-acetyltransferase	Toxin
CFI09_RS27440	36682..37174	-	493	Protein_42	hypothetical protein	-
CFI09_RS27685	37202..37448	+	247	Protein_43	DNA-binding protein	-
CFI09_RS25945	37663..39435	+	1773	WP_101973916.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	paa / afaC-VII / exeG / stcE	1..191691	191691

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (54-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17645.45 Da
• Isoelectric Point: 10.1083

>T78813 WP_101973914.1 NZ_CP022280:36213-36698 [Escherichia coli]
MGGVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAFGATRTFVVCRKGTQQVVGFYSLATGSVNHIEATGNLRRNM
PDPIPVIILARLAVDASFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
R

Nucleotide

• Length: 486 bp

>T78813 NZ_CP022280:36213-36698 [Escherichia coli]
ATGGGAGGTGTAACAGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTTCTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCTTTCGGAGCCACCCGGACTTTTGTTGTATGCAGGAAAGGTACGC
AACAAGTAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAACCATATAGAAGCTACAGGCAATCTTCGGCGCAATATG
CCTGATCCTATCCCTGTCATTATACTTGCACGCCTGGCTGTTGATGCGTCATTCCGTGGTAAAGGGCTTGGTGCAGATTT
ACTGCATGATGCGGTTTGTCGTTGTTACAGAGTGGCCGAAAATATAGGTGTGCGTGCAATTATGGTTCATGCACTTACGG
AAAACGCAAAACAGTTTTATATTCACCATGGTTTTAAGCCATCAAAAACCCAGGTGCAAACATTGTTTCTTAAACTCCCC
CGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10915.58 Da
• Isoelectric Point: 4.7037

>AT78813 WP_101973913.1 NZ_CP022280:35941-36225 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRVLIDTAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEP
AIEKLLARKPQWEV

Nucleotide

• Length: 285 bp

>AT78813 NZ_CP022280:35941-36225 [Escherichia coli]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAACTCAATCTCAGAGCAAGAGAATCACAGCGGGTACTCATTGACAC
TGCTGCAGAAATTCTTCATAAGTCACGTACAGACTTCATTCTGGAAATGGCCTGTAAAGCTGCGGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCAGATGAGCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGAGGTGTAA