Detailed information of TA system TA078808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 8506..9065
• Replicon: plasmid pSTEC299_1
• Accession: NZ_CP022280
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: CFI09_RS25730
• Protein ID: WP_000421259.1
• Coordinates: 8506..8781 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CFI09_RS25735
• Protein ID: WP_033804286.1
• Coordinates: 8781..9065 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS25700	5929..6269	-	341	Protein_6	hypothetical protein	-
CFI09_RS25710	6619..7221	+	603	WP_101973898.1	proQ/FINO family protein	-
CFI09_RS25715	7324..7590	+	267	WP_101973981.1	hypothetical protein	-
CFI09_RS25725	8118..8399	-	282	WP_101973900.1	hypothetical protein	-
CFI09_RS25730	8506..8781	-	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFI09_RS25735	8781..9065	-	285	WP_033804286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CFI09_RS25750	9952..10323	+	372	WP_101973982.1	DUF305 domain-containing protein	-
CFI09_RS25755	10310..11374	+	1065	WP_101973902.1	hypothetical protein	-
CFI09_RS25760	11811..12569	+	759	WP_101973903.1	substrate-binding domain-containing protein	-
CFI09_RS25765	12660..13664	+	1005	WP_032334768.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	paa / afaC-VII / exeG / stcE	1..191691	191691
	-	flank	IS/Tn	-	-	12660..13664	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T78808 WP_000421259.1 NZ_CP022280:c8781-8506 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T78808 NZ_CP022280:c8781-8506 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTACGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10444.87 Da
• Isoelectric Point: 5.6558

>AT78808 WP_033804286.1 NZ_CP022280:c9065-8781 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT78808 NZ_CP022280:c9065-8781 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGATCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49