Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4005602..4006152 Replicon chromosome
Accession NZ_CP022279
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name ccdB Uniprot ID B7MNQ7
Locus tag CFI09_RS21130 Protein ID WP_001160958.1
Coordinates 4005602..4005916 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CFI09_RS21135 Protein ID WP_000125566.1
Coordinates 4005919..4006152 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS21105 4001277..4002563 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
CFI09_RS21110 4002563..4003552 + 990 WP_000241230.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CFI09_RS21115 4003549..4004370 + 822 WP_001065362.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CFI09_RS21120 4004373..4004750 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CFI09_RS21125 4004757..4005605 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CFI09_RS21130 4005602..4005916 - 315 WP_001160958.1 CcdB family protein Toxin
CFI09_RS21135 4005919..4006152 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CFI09_RS21140 4006238..4006717 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
CFI09_RS21145 4006909..4008771 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
CFI09_RS21150 4008764..4009294 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CFI09_RS21155 4009402..4010733 - 1332 WP_001183207.1 MFS transporter -
CFI09_RS21160 4010792..4011079 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11678.65 Da        Isoelectric Point: 8.0666

>T78799 WP_001160958.1 NZ_CP022279:c4005916-4005602 [Escherichia coli]
MQFTVYHSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLLKGALDFMLDGI

Download         Length: 315 bp

>T78799 NZ_CP022279:c4005916-4005602 [Escherichia coli]
ATGCAATTTACGGTATACCACAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGCTTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT78799 WP_000125566.1 NZ_CP022279:c4006152-4005919 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT78799 NZ_CP022279:c4006152-4005919 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376W198

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References