Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3550490..3551108 Replicon chromosome
Accession NZ_CP022279
Organism Escherichia coli strain STEC299

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CFI09_RS18880 Protein ID WP_001291435.1
Coordinates 3550890..3551108 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CFI09_RS18875 Protein ID WP_000344800.1
Coordinates 3550490..3550864 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFI09_RS18865 3545580..3546773 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CFI09_RS18870 3546796..3549945 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
CFI09_RS18875 3550490..3550864 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CFI09_RS18880 3550890..3551108 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CFI09_RS18885 3551281..3551832 + 552 WP_000102539.1 maltose O-acetyltransferase -
CFI09_RS18890 3551948..3552418 + 471 WP_000136192.1 YlaC family protein -
CFI09_RS18895 3552582..3554132 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CFI09_RS18900 3554174..3554527 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CFI09_RS18910 3554906..3555217 + 312 WP_000409908.1 MGMT family protein -
CFI09_RS18915 3555248..3555820 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78797 WP_001291435.1 NZ_CP022279:3550890-3551108 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T78797 NZ_CP022279:3550890-3551108 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT78797 WP_000344800.1 NZ_CP022279:3550490-3550864 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT78797 NZ_CP022279:3550490-3550864 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References