Detailed information of TA system TA078785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1854022..1854503
• Replicon: chromosome
• Accession: NZ_CP022279
• Organism: Escherichia coli strain STEC299

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: CFI09_RS09225
• Protein ID: WP_101973497.1
• Coordinates: 1854022..1854312 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: CFI09_RS09230
• Protein ID: WP_101973498.1
• Coordinates: 1854312..1854503 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFI09_RS09180	1849274..1849477	-	204	WP_087614020.1	DUF4224 domain-containing protein	-
CFI09_RS09185	1849536..1851971	-	2436	WP_101973493.1	exonuclease	-
CFI09_RS09190	1852067..1852255	-	189	WP_101973494.1	DUF1482 family protein	-
CFI09_RS09195	1852252..1852440	-	189	WP_101973495.1	cell division inhibitor	-
CFI09_RS27510	1852992..1853213	+	222	WP_000157196.1	hypothetical protein	-
CFI09_RS09215	1853203..1853583	-	381	WP_101973863.1	hypothetical protein	-
CFI09_RS09220	1853595..1853747	-	153	WP_101973496.1	DUF1391 family protein	-
CFI09_RS09225	1854022..1854312	-	291	WP_101973497.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFI09_RS09230	1854312..1854503	-	192	WP_101973498.1	antitoxin	Antitoxin
CFI09_RS09235	1854521..1855021	-	501	WP_101973499.1	helix-turn-helix transcriptional regulator	-
CFI09_RS09240	1855129..1855392	+	264	WP_101973500.1	helix-turn-helix domain-containing protein	-
CFI09_RS09245	1855389..1855814	+	426	WP_101973501.1	Rha family transcriptional regulator	-
CFI09_RS09250	1855837..1856805	+	969	WP_101973864.1	helix-turn-helix domain-containing protein	-
CFI09_RS09255	1856812..1857558	+	747	WP_101973502.1	ATP-binding protein	-
CFI09_RS09260	1857580..1858302	+	723	WP_101973503.1	DUF1627 domain-containing protein	-
CFI09_RS09265	1858335..1858616	+	282	WP_101973865.1	DNA-binding protein	-
CFI09_RS09270	1858613..1858909	+	297	WP_101973504.1	DUF4406 domain-containing protein	-
CFI09_RS09275	1858906..1859178	+	273	Protein_1706	ead/Ea22-like family protein	-
CFI09_RS27515	1859161..1859303	+	143	Protein_1707	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtP / ybtQ / ybtX / ybtS	1839226..1948347	109121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11143.97 Da
• Isoelectric Point: 9.9855

>T78785 WP_101973497.1 NZ_CP022279:c1854312-1854022 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPHAARRLKIRIETSVLSLTEHPYLYPPSERAPGLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T78785 NZ_CP022279:c1854312-1854022 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCGCGTGATGATTTGCGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCA
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTACCTGTATCCACCAAGCG
AAAGAGCTCCAGGCCTTCGTGAGATAGTGACCCATCCTAACTACATAATACTTTATCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTCCACTCACGAAGACAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6953.81 Da
• Isoelectric Point: 4.1015

>AT78785 WP_101973498.1 NZ_CP022279:c1854503-1854312 [Escherichia coli]
MSTALSPIISEFETIEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEIENLIAQIAATNRSV

Nucleotide

• Length: 192 bp

>AT78785 NZ_CP022279:c1854503-1854312 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCAGAATTTGAAACAATCGAACAAGAAAACAGCTATAACGAATGGTTACGCGC
AAAAGTAGCCGCAAGCCTTGCTGATCCACGTCCTGCTATTCCACATGATGAAGTTATGGCAGAAATTGAAAACCTTATTG
CTCAAATAGCTGCGACGAACAGGAGTGTGTGA