Detailed information of TA system TA078767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 17696..18207
• Replicon: plasmid pCKPC18-1
• Accession: NZ_CP022276
• Organism: Citrobacter freundii strain 18-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W0NIW1
• Locus tag: CFA70_RS28520
• Protein ID: WP_020956903.1
• Coordinates: 17932..18207 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CFA70_RS28515
• Protein ID: WP_020956904.1
• Coordinates: 17696..17920 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFA70_RS28475	14273..14479	-	207	Protein_16	hypothetical protein	-
CFA70_RS28480	14587..15111	+	525	WP_020956910.1	J domain-containing protein	-
CFA70_RS28485	15202..15744	+	543	WP_020956909.1	hypothetical protein	-
CFA70_RS28490	15741..15986	+	246	WP_020956908.1	hypothetical protein	-
CFA70_RS28495	15983..16204	+	222	WP_020956907.1	helix-turn-helix domain-containing protein	-
CFA70_RS28500	16209..16490	+	282	WP_088912116.1	hypothetical protein	-
CFA70_RS28505	16874..17086	+	213	WP_020956906.1	hypothetical protein	-
CFA70_RS28510	17132..17392	+	261	WP_070749909.1	hypothetical protein	-
CFA70_RS28515	17696..17920	+	225	WP_020956904.1	hypothetical protein	Antitoxin
CFA70_RS28520	17932..18207	+	276	WP_020956903.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFA70_RS28525	18229..18495	+	267	WP_020956902.1	hypothetical protein	-
CFA70_RS28530	18936..19490	+	555	WP_020956901.1	hypothetical protein	-
CFA70_RS28535	19492..20682	+	1191	WP_041937886.1	relaxase/mobilization nuclease domain-containing protein	-
CFA70_RS28540	20712..21038	-	327	WP_030003464.1	helix-turn-helix domain-containing protein	-
CFA70_RS28545	21258..21896	+	639	WP_020956900.1	lytic transglycosylase domain-containing protein	-
CFA70_RS28550	21883..22170	+	288	WP_020956899.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..47164	47164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-88)

Antitoxin

• Protein of unknown function DUF6290 (6-72)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10922.82 Da
• Isoelectric Point: 10.6500

>T78767 WP_020956903.1 NZ_CP022276:17932-18207 [Citrobacter freundii]
MTRYRTEYTKRFVKEFKKIDTVHQRVIQKYIEAHINNALNPRLTGKILKGDKAEFIRWRIGDYRLIATIEDDKLLVIALS
VGHRKDVYKKF

Nucleotide

• Length: 276 bp

>T78767 NZ_CP022276:17932-18207 [Citrobacter freundii]
ATGACACGCTACCGGACAGAATACACAAAACGGTTCGTCAAAGAGTTTAAGAAAATTGACACGGTACACCAGAGGGTAAT
TCAGAAGTACATTGAGGCGCACATCAACAACGCACTCAATCCGCGCTTGACGGGTAAAATCCTGAAAGGAGATAAAGCCG
AATTTATCCGCTGGCGTATCGGTGATTACCGTCTGATTGCCACTATTGAAGACGATAAATTGCTGGTCATTGCGTTAAGC
GTGGGACACCGCAAAGACGTGTACAAAAAGTTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8535.52 Da
• Isoelectric Point: 4.3313

>AT78767 WP_020956904.1 NZ_CP022276:17696-17920 [Citrobacter freundii]
MNKNMTKAIRLNDEDFNLFESYANAKGITFSELCKSAVREKIEDELDLQCANESYADYLSDKTTISHDELFKSM

Nucleotide

• Length: 225 bp

>AT78767 NZ_CP022276:17696-17920 [Citrobacter freundii]
ATGAACAAAAACATGACGAAAGCGATCCGGTTGAATGACGAAGATTTTAACCTGTTTGAGTCTTACGCCAACGCAAAAGG
GATAACCTTCAGCGAATTGTGCAAAAGCGCAGTACGGGAAAAAATCGAGGACGAATTAGATTTGCAATGTGCTAATGAAA
GCTACGCGGATTATCTCAGCGACAAAACGACAATCAGCCACGACGAACTGTTTAAATCTATGTGA

Structures

Toxin

• AlphaFold DB: A0A384ZT31