Detailed information of TA system TA078766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 30241..30776
• Replicon: plasmid pBKPC18-1
• Accession: NZ_CP022275
• Organism: Citrobacter freundii strain 18-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: CFA70_RS27745
• Protein ID: WP_032693963.1
• Coordinates: 30489..30776 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CFA70_RS27740
• Protein ID: WP_032693964.1
• Coordinates: 30241..30492 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFA70_RS27730	27252..28670	+	1419	WP_137922721.1	multidrug efflux transporter outer membrane subunit TOprJ1	-
CFA70_RS29180	28840..28989	-	150	WP_155275771.1	hypothetical protein	-
CFA70_RS27735	29100..30074	+	975	Protein_31	translesion error-prone DNA polymerase V subunit UmuC	-
CFA70_RS27740	30241..30492	+	252	WP_032693964.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CFA70_RS27745	30489..30776	+	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFA70_RS27750	30841..31047	+	207	WP_088903433.1	hypothetical protein	-
CFA70_RS27760	31862..32893	-	1032	WP_001752311.1	IS630-like element ISEc33 family transposase	-
CFA70_RS27765	32979..33449	+	471	WP_088903435.1	hypothetical protein	-
CFA70_RS27770	33725..34117	-	393	WP_080884174.1	plasmid partitioning/stability family protein	-
CFA70_RS27775	34121..35095	-	975	WP_080884175.1	StbA family protein	-
CFA70_RS27780	35335..35709	-	375	WP_088903436.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..144825	144825
	-	flank	IS/Tn	-	-	31862..32893	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T78766 WP_032693963.1 NZ_CP022275:30489-30776 [Citrobacter freundii]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T78766 NZ_CP022275:30489-30776 [Citrobacter freundii]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAGGAATGGCAAAAGCTGGATAAGGCCATTCAGCAACAGTTTGC
GAAGAAGCTGAAGAAGTGCTGTGACGAACCACATATCCCCTCAGCAAAGCTGAGAGGGATAAAGGACTGCTATAAAATTA
AACTTCGCGCGTCAGGCTTTCGTCTGGTCTATCAGGTAATAGATGATCAACTGATTATTGCTGTTGTGGCTGTCGGAAAG
CGTGAACGTAGTGACGTGTACAATCTGGCCAGCGAACGGATGAGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9202.49 Da
• Isoelectric Point: 4.0785

>AT78766 WP_032693964.1 NZ_CP022275:30241-30492 [Citrobacter freundii]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQRLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT78766 NZ_CP022275:30241-30492 [Citrobacter freundii]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCTATTCTGAACCGTAATCAGCCCGCTTTTTACTGCATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTTTCAGAACGCAGTAACCAGCGACTGCTTGACGTGGATCTGGAC
ACATATCTATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2