Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 140327..141063 Replicon plasmid pA18-1
Accession NZ_CP022274
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CFA70_RS27500 Protein ID WP_003026803.1
Coordinates 140581..141063 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CFA70_RS27495 Protein ID WP_003026799.1
Coordinates 140327..140593 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS27470 135721..136725 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
CFA70_RS27475 137382..137588 + 207 WP_032736819.1 hypothetical protein -
CFA70_RS27480 137603..138298 + 696 WP_032736820.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
CFA70_RS27485 138499..139610 + 1112 WP_088903424.1 IS3 family transposase -
CFA70_RS27490 139590..140057 - 468 WP_177339880.1 hypothetical protein -
CFA70_RS27495 140327..140593 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CFA70_RS27500 140581..141063 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CFA70_RS27505 141264..142667 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
CFA70_RS27510 142696..143328 - 633 WP_001567369.1 hypothetical protein -
CFA70_RS27515 143554..144900 + 1347 WP_077253535.1 ISNCY family transposase -
CFA70_RS27520 144949..145344 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..159655 159655
- inside IScluster/Tn - - 133678..146773 13095


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T78765 WP_003026803.1 NZ_CP022274:140581-141063 [Citrobacter freundii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T78765 NZ_CP022274:140581-141063 [Citrobacter freundii]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT78765 WP_003026799.1 NZ_CP022274:140327-140593 [Citrobacter freundii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT78765 NZ_CP022274:140327-140593 [Citrobacter freundii]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References